# US 10,865,372 B2 · Neuron cultivation device, neuron cultivating method, cultivated neuron, analysis and identification of protein in axon bundle, and usage of cultivated neuron > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,865,372 B2 - Document ID: US10865372B2 - Status: Granted - Kind: B2 - Application number: 16/094,296 - Filed: 2017-02-01 - Published: 2019-05-02 - Granted: 2020-12-15 - Earliest claimed: 2016-04-28 - Assignees: The Foundation for the Promotion of Industrial Science - Applicants: The Foundation for the Promotion of Industrial Science - Inventors: Teruo Fujii; Yoshiho Ikeuchi; Shohei Kaneda; Jiro Kawada - Web page: https://patentgrep.com/patent/US10865372B2 ## CPC - C12M 21/08: {for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00)} - C12M 3/00: Tissue, human, animal or plant cell, or virus culture apparatus - C12M 23/12: {Well or multiwell plates (C12M25/04 takes precedence)} - C12M 23/16: {Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093)} - C12M 23/34: {Internal compartments or partitions} - C12M 23/44: {Multiple separable units; Modules} - C12M 23/58: {Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00)} - C12M 35/08: {Chemical, biochemical or biological means, e.g. plasma jet, co-culture} - C12M 37/04: {Seals} - C12N 5/0619: {Neurons} - C12N 5/0658: {Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts} - C12N 5/0697: {Artificial constructs associating cells of different lineages, e.g. tissue equivalents (blood vessels C12N5/0691)} - C12Q 1/02: involving viable microorganisms - G01N 33/5005: {involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025)} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract An object is to provide a neuron cultivation device which promptly develops bundles of axons extending from neurons in vitro. A device for cultivating neuron with axon, the device comprising a cultivation plate and a plurality of modules arranged in the cultivation plate. Each of the modules includes at least one of first chambers receivable of cell bodies of neurons at least one of second chambers, and at least one of channels receivable of a bundle of axon extended from the cell bodies. The channels connect the first chambers and the second chambers. Bottom ends of the first chambers, the second chambers and the channels are closed and top ends of the first chambers and the second chambers are open. ## Claims ### Claim 1 An aggregated neuron body consisting of a plurality of neurons with cell bodies derived from human iPS cells and axons extended from the cell bodies, the aggregated neuron body comprising: a spheroid and a bundle of axons of 1 mm or more in length extended from the spheroid in one direction, wherein the spheroid is an aggregation of a plurality of the cell bodies, and wherein the bundle of axons is a bundle of a plurality of the axons, glial cells being non-existent in the bundle of axons to a degree that a marker indicating existence of the glial cells is negative, wherein the marker is Glia Fibrillary Acidic Protein (GFAP). ### Claim 2 (depends on 1) The aggregated neuron body according to claim 1, wherein the bundle of axons is 4.5 mm or more in the length. ### Claim 3 (depends on 2) The aggregated neuron body according to claim 2, wherein the spheroid includes a glia cell. ### Claim 4 (depends on 2) The aggregated neuron body according to claim 2, wherein the aggregated neuron body is isolated. ### Claim 5 (depends on 2) The aggregated neuron body according to claim 2, wherein the aggregated neuron body is motoneuron. ### Claim 6 (depends on 5) The aggregated neuron body according to claim 5, wherein the bundles of axons conjugate at the axon terminal with skeletal muscle cells. ### Claim 7 (depends on 1) The aggregated neuron body according to claim 1, wherein the spheroid includes a glia cell. ### Claim 8 (depends on 7) The aggregated neuron body according to claim 7, wherein the aggregated neuron body is isolated. ### Claim 9 (depends on 7) The aggregated neuron body according to claim 7, wherein the aggregated neuron body is motoneuron. ### Claim 10 (depends on 9) The aggregated neuron body according to claim 9, wherein the bundles of axons conjugate at the axon terminal with skeletal muscle cells. ### Claim 11 (depends on 1) The aggregated neuron body according to claim 1, wherein the aggregated neuron body is isolated. ### Claim 12 (depends on 11) The aggregated neuron body according to claim 11, wherein the aggregated neuron body is motoneuron. ### Claim 13 (depends on 12) The aggregated neuron body according to claim 12, wherein the bundles of axons conjugate at the axon terminal with skeletal muscle cells. ### Claim 14 (depends on 1) The aggregated neuron body according to claim 1, wherein the aggregated neuron body is motoneuron. ### Claim 15 (depends on 14) The aggregated neuron body according to claim 14, wherein the bundles of axons conjugate at the axon terminal with skeletal muscle cells.