# US 10,865,371 B2 · Large scale mixotrophic production systems > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,865,371 B2 - Document ID: US10865371B2 - Status: Granted - Kind: B2 - Application number: 14/822,716 - Filed: 2015-08-10 - Published: 2015-12-03 - Granted: 2020-12-15 - Earliest claimed: 2013-03-15 - Assignees: Heliae Development, LLC - Applicants: Heliae Development LLC - Inventors: Eneko Ganuza Taberna; Thomas Adame; Anna Lee Tonkovich; Adriano Galvez; Timothy Sullivan - Web page: https://patentgrep.com/patent/US10865371B2 ## CPC - C12M 21/02: {Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12)} - C12M 23/18: {Open ponds; Greenhouse type or underground installations} - C12M 23/34: {Internal compartments or partitions} - C12M 23/38: {Caps; Covers; Plugs; Pouring means} - C12M 27/06: {with horizontal or inclined stirrer shaft or axis} - C12M 41/24: {inside the vessel} - C12M 41/26: {of pH} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract Bioreactor systems for culturing mixotrophic microorganisms in open cultures on a large scale are disclosed herein. Embodiments of the system comprise organic carbon delivery systems and submersible thrusters suspended on adjustable support structures. ## Claims ### Claim 1 A mixotrophic bioreactor system, comprising: a. An open raceway pond comprising: i. An inner volume; ii. Two straight away portions separated by a center wall and bounded by straight outer walls and a floor; and iii. Two U-bend portions connecting the two straight away portions to form a continuous looped flow path, and bounded by a curved outer wall and a floor; iv. Wherein the inner volume has a consistent depth along the entire looped flow path; b. At least one submersible thruster disposed within the inner volume a distance from the floor, wherein the at least one thruster is positioned at a U-bend exit and configured to maximize thrust in a straight line flow of an aqueous liquid culture through the straight away portions and add momentum to the aqueous liquid culture along the looped flow path as it exits the U-bend portions of the looped flow path where some velocity of the aqueous liquid culture may be lost due to change in flow direction; c. At least one support structure coupled to at least one of the center wall and an outer wall, the support structure suspending the at least one submersible thruster from above at a position within the inner volume of the open raceway pond, wherein the at least one support structure is configured to allow adjustment of the position of the at least one submersible thruster vertically, horizontally, or both; and comprising a distance from the floor to the at least one submersible thruster of 10-50% of a height of an aqueous liquid culture volume in the inner volume of the open raceway pond; and d. At least one organic carbon delivery device comprising a pH auxostat system with an outlet positioned to deliver an organic carbon source to the inner volume of the open raceway pond. ### Claim 2 (depends on 1) The mixotrophic bioreactor system of claim 1, wherein the at least one thruster comprises multiple submersible thrusters and the thrusters are disposed within 20% of the U-bend end of the straight away portions. ### Claim 3 (depends on 2) The mixotrophic bioreactor system of claim 2, wherein the width of the open raceway pond is 3 to 12 meters. ### Claim 4 (depends on 2) The mixotrophic bioreactor system of claim 2, wherein the system comprises a culture of microalgae selected from the group consisting of Chlorella, Thraustochytrids, and combinations thereof. ### Claim 5 (depends on 4) The mixotrophic bioreactor system of claim 4, wherein the culture has a dissolved oxygen concentration that varies by 10% or less throughout the culture. ### Claim 6 (depends on 1) The mixotrophic bioreactor system of claim 1, wherein the at least one submersible thruster comprises multiple submersible thrusters positioned parallel to each other within the inner volume of the open raceway pond. ### Claim 7 (depends on 1) The mixotrophic bioreactor system of claim 1, wherein the at least one submersible thruster comprises multiple submersible thrusters positioned in a staggered arrangement with relation to each other within the inner volume of the open raceway pond. ### Claim 8 (depends on 1) The mixotrophic bioreactor system of claim 1, wherein the at least one submersible thruster comprises multiple submersible thrusters positioned at spaced intervals within the inner volume of the open raceway pond. ### Claim 9 (depends on 1) The mixotrophic bioreactor system of claim 1, wherein the at least one submersible thruster is disposed between the center wall and the outer wall of at least one of the straight away portions. ### Claim 10 (depends on 1) The mixotrophic bioreactor system of claim 1, wherein the at least one submersible thruster is oriented to circulate a fluid medium through the continuous loop of the open raceway pond. ### Claim 11 (depends on 1) The mixotrophic bioreactor system of claim 1, further comprising at least one heat exchanger. ### Claim 12 (depends on 11) The mixotrophic bioreactor system of claim 11, wherein the at least one heat exchanger is disposed within at least one from the group consisting of the outer walls, the center wall, the floor, and under the floor of the open raceway pond. ### Claim 13 (depends on 1) The mixotrophic bioreactor system of claim 1, further comprising a cover over at least part of the open raceway pond. ### Claim 14 (depends on 1) The mixotrophic bioreactor system of claim 1, further comprising at least one light source selected from the group consisting of natural light and an artificial lighting device. ### Claim 15 (depends on 1) The mixotrophic bioreactor system of claim 1, further comprising at least one arched turning vane disposed within each U-bend portion. ### Claim 16 (depends on 15) The mixotrophic bioreactor system of claim 15, wherein the at least one arched turning vane comprises a symmetrical or asymmetrical curve profile. ### Claim 17 A mixotrophic bioreactor system, comprising: a. An open raceway pond comprising: i. A floor; ii. Two straight away portions separated by a center wall and bounded by straight outer walls and the floor; and iii. Two U-bend portions connecting the two straight away portions to form a continuous looped flow path, and bounded by a curved outer wall and the floor; iv. Wherein the floor is flat to create an inner volume that has a consistent depth along the entire looped flow path; b. At least one submersible thruster disposed within the inner volume a distance from the floor, wherein the at least one thruster is positioned at a U-bend exit and configured to maximize thrust in a straight line flow of an aqueous liquid culture through the straight away portions and add momentum to the aqueous liquid culture along the looped flow path as it exits the U-bend portions of the looped flow path where some velocity of the aqueous liquid culture may be lost due to change in flow direction; c. At least one support structure coupled to at least one of the center wall and an outer wall, the support structure suspending the at least one submersible thruster from above at a position within the inner volume of the open raceway pond wherein the at least one support structure is configured to allow adjustment of the position of the at least one submersible thruster vertically, horizontally, or both; and comprising a distance from the floor to the at least one submersible thruster of 10-50% of a height of an aqueous liquid culture volume in the inner volume of the open raceway pond; and d. At least one organic carbon delivery device comprising a pH auxostat system with an outlet positioned to deliver an organic carbon source to the inner volume of the open raceway pond.