# US 10,865,366 B2 · Lipase variants and polynucleotides encoding same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,865,366 B2 - Document ID: US10865366B2 - Status: Granted - Kind: B2 - Application number: 15/513,435 - Filed: 2015-09-28 - Published: 2017-10-26 - Granted: 2020-12-15 - Earliest claimed: 2014-09-29 - Assignees: Novozymes A/S - Applicants: Novozymes A/S - Inventors: Carsten Hoerslev Hansen; Jesper Vind; Luise Erlandsen; Bitten Plesner; Anna Catharina Olsen Stenholm - Web page: https://patentgrep.com/patent/US10865366B2 ## CPC - C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase} - C11D 3/38627: {containing lipase} - C11D 3/38681: {Chemically modified or immobilised enzymes} - C11D 3/392: {Heterocyclic compounds, e.g. cyclic imides or lactames} - C12N 9/16: acting on ester bonds (3.1) - C12N 9/20: Triglyceride splitting, e.g. by means of lipase - C12N 9/00: Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) - C12Y 301/00: Hydrolases acting on ester bonds (3.1) - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Y: ENZYMES ## Abstract The present invention relates to lipase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. ## Claims ### Claim 1 A lipase variant, wherein the variant has lipase activity and comprises an amino acid sequence that has at least 90% but less than 100% sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the variant comprises a substitution of asparagine with glutamine at the position corresponding to position 33 of SEQ ID NO: 2, a substitution of glycine with glutamine at the position corresponding to position 91 of SEQ ID NO: 2, a substitution of glutamate with glutamine at the position corresponding to position 210 of SEQ ID NO: 2, a substitution of isoleucine with alanine at the position corresponding to position 255 of SEQ ID NO: 2, and a substitution of proline with a different amino acid at the position corresponding to position 256 of SEQ ID NO: 2, wherein said different amino acid is not lysine. ### Claim 2 (depends on 1) The variant of claim 1, wherein the variant further comprises a substitution at positions of SEQ ID NO: 2 corresponding to position 231 and/or 233. ### Claim 3 (depends on 2) The variant of claim 2, wherein the substitution at position: 231 is H, or K; 233 is H, or K; or 256 is A, C, D, E, F, G, H, I, L, M, N, Q, R, S, T, V, W, or Y. ### Claim 4 (depends on 1) The variant of claim 1, wherein the variant further comprises substitutions at positions of SEQ ID NO: 2 corresponding to positions 231 and 233. ### Claim 5 (depends on 1) The variant of claim 1, further comprising one or more of the substitutions N33Q, G91Q, T143A, or A150G, wherein the amino acid numbering corresponds to the amino acid sequence of SEQ ID NO: 2. ### Claim 6 (depends on 1) The variant of claim 1, which has a reduced relative odor release as compared with SEQ ID NO: 2. ### Claim 7 (depends on 1) A composition comprising the variant of claim 1. ### Claim 8 (depends on 7) A method of cleaning a surface comprising contacting the surface with the composition of claim 7. ### Claim 9 (depends on 1) A polynucleotide comprising a nucleotide sequence encoding the variant of claim 1. ### Claim 10 (depends on 9) A nucleic acid construct comprising the polynucleotide of claim 9. ### Claim 11 (depends on 9) An expression vector comprising the polynucleotide of claim 9. ### Claim 12 (depends on 9) An isolated host cell comprising the polynucleotide of claim 9. ### Claim 13 (depends on 12) A method of producing a lipase variant, comprising: a. cultivating the host cell of claim 12 under conditions suitable for expression of the variant; and b. recovering the variant.