# US 10,865,316 B2 · Conotoxin peptides for use in biofouling deterrence

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- Number: US 10,865,316 B2
- Document ID: US10865316B2
- Status: Granted
- Kind: B2
- Application number: 16/685,101
- Filed: 2019-11-15
- Published: 2020-03-05
- Granted: 2020-12-15
- Earliest claimed: 2015-05-22
- Assignees: Clemson University Research Foundation
- Applicants: Clemson University Research Foundation
- Inventors: Andrew Mount; Bin San Chan; Mary Beth Johnstone
- Web page: https://patentgrep.com/patent/US10865316B2

## CPC

- C09D 5/1625: {organic}
- C07K 7/08: having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68)
- C07K 14/43504: {from invertebrates}
- C02F 2303/20: Prevention of biofouling
- C09D: COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR (cosmetics A61K; processes for applying liquids or other fluent materials to surfaces, in general, B05D; staining wood B27K5/02; glazes or vitreous enamels C03C; natural resins, French polish, drying-oils, driers, turpentine, per se, C09F; polishing compositions other than French polish, ski waxes C09G; adhesives or use of materials as adhesives C09J; materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12; processes for the electrolytic or electrophoretic production of coatings C25D)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)

## Abstract

Materials and methods for prevention of biofouling that incorporate the presence of a conotoxin peptide on a surface are described. The conotoxin peptide is either directly or indirectly adhered to the surface and interferes with the ability of biofouling organisms to settle on the surface.

## Claims

### Claim 1

A method for preventing biofouling, the method comprising directly or indirectly adhering a conotoxin peptide to an aquatic surface.

### Claim 2 (depends on 1)

The method of claim 1, the method further comprising pretreating the surface to form reactive functionality on the surface.

### Claim 3 (depends on 2)

The method of claim 2, wherein the reactive functionality is a product of a reaction between an azide and an alkyne.

### Claim 4 (depends on 1)

The method of claim 1, comprising applying a coating composition to the surface, the coating composition comprising the conotoxin peptide.

### Claim 5 (depends on 4)

The method of claim 4, wherein the coating composition is a marine paint.

### Claim 6 (depends on 4)

The method of claim 4, the conotoxin peptide being bonded to a component of the coating composition.

### Claim 7 (depends on 1)

The method of claim 1, the conotoxin peptide being bonded to the aquatic surface via a linking agent.

### Claim 8 (depends on 1)

The method of claim 1, wherein the surface comprises metal, wood, concrete, glass, or an organic polymer.

### Claim 9 (depends on 1)

The method of claim 1, wherein the conotoxin peptide is a χ-conotoxin peptide.

### Claim 10 (depends on 1)

The method of claim 1, wherein the conotoxin peptide comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, or SEQ ID NO: 13.

### Claim 11 (depends on 1)

The method of claim 1, wherein the surface is the surface of a ship or a fixed or floating marine structure.

### Claim 12 (depends on 1)

The method of claim 1, wherein the conotoxin peptide is directly or indirectly adhered to the surface in an amount of about 1014 peptides per square centimeter or greater.

### Claim 13

A method for preventing biofouling, the method comprising applying a coating to a surface, the coating comprising a conotoxin peptide bonded to a component of the coating composition via a polymeric linking agent between the component and the conotoxin peptide, the conotoxin peptide being covalently bonded to the polymeric linking agent.

### Claim 14 (depends on 13)

The method of claim 13, wherein the conotoxin peptide is covalently bonded to the polymeric linking agent via an N-terminus of the conotoxin peptide.

### Claim 15 (depends on 13)

The method of claim 13, wherein the polymeric linking agent is a peptidic polymeric linking agent.

### Claim 16 (depends on 15)

The method of claim 15, wherein the peptidic polymeric linking agent comprises a glycyl chain.

### Claim 17 (depends on 13)

The method of claim 13, wherein the polymeric linking agent is bonded to the component via a reaction product of a terminal functionality of the polymeric linking agent, the terminal functionality being selected from the group consisting of thiol, primary amine, secondary amine, nitrile, aldehyde, imidazole, azide, halide, polyhexamethylene dithiocarbonate, hydrogen, hydroxyl, carboxylic acid, carboxylic ester, and carboxamide.

### Claim 18 (depends on 13)

The method of claim 13, wherein the polymeric linking agent is selected from the group consisting of a polyfunctional epoxide, a polyisocyanate, a polyhydric alcohol, a carbodiimide, a diamine, a diaminoalkane, and a polyfunctional carboxylic acid.

### Claim 19 (depends on 13)

The method of claim 13, wherein the conotoxin peptide is a χ-conotoxin peptide.

### Claim 20 (depends on 13)

The method of claim 13, wherein the conotoxin peptide comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, or SEQ ID NO: 13.