# US 10,865,237 B2 · Nucleic acids encoding anti-Factor P antibodies

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- Number: US 10,865,237 B2
- Document ID: US10865237B2
- Status: Granted
- Kind: B2
- Application number: 15/973,225
- Filed: 2018-05-07
- Published: 2018-11-15
- Granted: 2020-12-15
- Earliest claimed: 2011-12-21
- Assignees: Novartis AG
- Applicants: Novartis AG
- Inventors: Leslie Ngozi Anuna Johnson; Ute Jaeger; Yong-In Kim; Christian Carsten Silvester Kunz; Igor Splawski; Michael Roguska; Joy Ghosh; Barbara Brannetti; Michael Stefanidakis; Sha-Mei Liao
- Web page: https://patentgrep.com/patent/US10865237B2

## CPC

- C07K 16/18: against material from animals or humans
- A61K 39/3955: {against proteinaceous materials, e.g. enzymes, hormones, lymphokines}
- A61P 27/02: Ophthalmic agents
- A61P 37/06: Immunosuppressants, e.g. drugs for graft rejection
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C07K 16/24: against cytokines, lymphokines or interferons
- G01N 33/6863: {Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors}
- A61K 2039/505: {comprising antibodies}
- A61K 2039/507: {Comprising a combination of two or more separate antibodies}
- C07K 2317/21: from primates, e.g. man
- C07K 2317/30: characterized by aspects of specificity or valency
- C07K 2317/33: Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
- C07K 2317/40: characterized by post-translational modification
- C07K 2317/55: Fab or Fab'
- C07K 2317/565: Complementarity determining region [CDR]
- C07K 2317/76: Antagonist effect on antigen, e.g. neutralization or inhibition of binding
- C07K 2317/92: Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to antibodies or antigen binding fragments thereof that bind to complement Factor P and used thereof as well as combinations of anti-Factor P antibodies with antibodies or antigen binding fragments thereof that bind to complement component 5 (C5).

## Claims

### Claim 1

An isolated nucleic acid molecule comprising a nucleotide sequence encoding an antibody or antigen-binding fragment comprising:
a) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 1, 2, and 3, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 4, 5, and 6, respectively;
b) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 15, 16, and 17, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 18, 19, and 20, respectively;
c) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 29, 30, and 31, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 32, 33, and 34, respectively;
d) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 43, 44, and 45, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 46, 47, and 48, respectively;
e) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 57, 58, and 59, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 60, 61, and 62, respectively;
f) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 71, 72, and 73, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 74, 75, and 76, respectively;
g) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 85, 86, and 87, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 88, 89, and 90, respectively;
h) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 99, 100, and 101, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 102, 103, and 104, respectively;
i) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 113, 114, and 115, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 116, 117, and 118, respectively;
j) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 127, 128, and 129, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 130, 131, and 132, respectively;
k) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 141, 142, and 143, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 144, 145, and 146, respectively;
l) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 155, 156, and 157, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 158, 159, and 160, respectively;
m) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 169, 170, and 171, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 172, 173, and 174, respectively;
n) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 183, 184, and 185, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 186, 187, and 188, respectively;
o) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 197, 198, and 199, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 200, 201, and 202, respectively;
p) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 211, 212, and 213, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 214, 215, and 216, respectively;
q) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 225, 226, and 227, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 228, 229, and 230, respectively;
r) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 239, 240, and 241, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 242, 243, and 244, respectively;
s) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 253, 254, and 255, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 256, 257, and 258, respectively; or
t) heavy chain variable region HCDR1, HCDR2, and HCDR3 as set forth in SEQ ID NOs: 267, 268, and 269, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 270, 271, and 272, respectively.

### Claim 2

An isolated nucleic acid molecule encoding a polypeptide comprising a heavy chain variable region comprising SEQ ID NO: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259, or 273.

### Claim 3 (depends on 2)

The nucleic acid molecule of claim 2, wherein said nucleic acid molecule has 100% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 11, 25, 39, 53, 67, 81, 95, 109, 123, 137, 151, 165, 179, 193, 207, 221, 235, 249, 263, and 277.

### Claim 4

An isolated nucleic acid molecule encoding a polypeptide comprising a light chain variable region comprising SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274.

### Claim 5 (depends on 4)

The nucleic acid molecule of claim 4, wherein said nucleic acid molecule has 100% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 12, 26, 40, 54, 68, 82, 96, 110, 124, 138, 152, 166, 180, 194, 208, 222, 236, 250, 264, and 278.

### Claim 6

An isolated nucleic acid molecule encoding a polypeptide comprising a heavy chain comprising SEQ ID NO: 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219, 233, 247, 261, or 275.

### Claim 7 (depends on 6)

The nucleic acid molecule of claim 6, wherein said nucleic acid molecule has 100% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 13, 27, 41, 55, 69, 83, 97, 111, 125, 139, 153, 167, 181, 195, 209, 223, 237, 251, 265, and 279.

### Claim 8

An isolated nucleic acid molecule encoding a polypeptide comprising a light chain comprising SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, or 276.

### Claim 9 (depends on 8)

The nucleic acid molecule of claim 8, wherein said nucleic acid molecule has 100% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 14, 28, 42, 56, 70, 84, 98, 112, 126, 140, 154, 168, 182, 196, 210, 224, 238, 252, 266, and 280.

### Claim 10 (depends on 1)

A vector comprising the nucleic acid molecule of claim 1.

### Claim 11 (depends on 10)

An isolated host cell comprising the vector of claim 10.