# US 10,864,549 B2 · Half-coating method for nanoparticles

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- Number: US 10,864,549 B2
- Document ID: US10864549B2
- Status: Granted
- Kind: B2
- Application number: 16/085,891
- Filed: 2017-02-15
- Published: 2019-04-25
- Granted: 2020-12-15
- Earliest claimed: 2016-03-17
- Assignees: Sogang University Research Foundation
- Applicants: Sogang University Research Foundation
- Inventors: Jeong Woo Choi; Jin Ho Yoon; Sang Uk Kim
- Web page: https://patentgrep.com/patent/US10864549B2

## CPC

- C12Q 1/6818: involving interaction of two or more labels, e.g. resonant energy transfer
- B05D 3/104: {Pretreatment of other substrates}
- B05D 3/107: {Post-treatment of applied coatings}
- B22F 1/054: Nanosized particles
- B22F 1/102: Metallic powder coated with organic material
- C09D 7/00: Features of coating compositions, not provided for in group C09D5/00 (driers C09F9/00); Processes for incorporating ingredients in coating compositions
- C12Y 301/00: Hydrolases acting on ester bonds (3.1)
- B82Y 5/00: Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- B82Y 40/00: Manufacture or treatment of nanostructures
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- B05D: PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL (conveying articles or workpieces through baths of liquid B65G, e.g. B65G49/02)
- B22F: WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER (making alloys by powder metallurgy C22C); APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- C09D: COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR (cosmetics A61K; processes for applying liquids or other fluent materials to surfaces, in general, B05D; staining wood B27K5/02; glazes or vitreous enamels C03C; natural resins, French polish, drying-oils, driers, turpentine, per se, C09F; polishing compositions other than French polish, ski waxes C09G; adhesives or use of materials as adhesives C09J; materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12; processes for the electrolytic or electrophoretic production of coatings C25D)
- C12Y: ENZYMES
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES

## Abstract

The present invention provides a method and a kit for half coating nanoparticles. According to the present invention, nanoparticles can be half coated in a relatively simple and economical process that can replace complicated processes, such as chemical synthesis, of a conventional Janus nanoparticle-preparing method.

## Claims

### Claim 1

A kit comprising:
(a) a substrate;
(b) a first oligonucleotide self-assembled on the substrate;
(c) a second oligonucleotide partially hybridized with a terminal of the first oligonucleotide;
(d) single-stranded DNA nuclease or a double-stranded DNA nuclease; and
(e) nanoparticles half-coated with a chemical substance complex, wherein the chemical substance complex is cucibit [8] Uril (CB), Trp Gly Gly (WGG peptide), and 5,5′Bis(mercaptomethyl)-2,2′-bipyridine.

### Claim 2 (depends on 1)

The kit of claim 1, wherein the substrate is formed of a metal, a metal oxide, glass, a ceramic, quartz, silicon, a semiconductor, a Si/SiO2 wafer, germanium, gallium arsenide, carbon, carbon nanotubes, a polymer, Sepharose, or agarose.

### Claim 3 (depends on 1)

The kit of claim 1, wherein the first oligonucleotide is 10-100 bp (base pair) in length.

### Claim 4 (depends on 1)

The kit of claim 1, wherein the terminal of the first oligonucleotide is 3′-terminal or 5′-terminal.

### Claim 5 (depends on 1)

The kit of claim 1, wherein the second oligonucleotide is 10-100 bp (base pair) in length.

### Claim 6 (depends on 1)

The kit of claim 1, wherein the second oligonucleotide is hybridized with a terminal region of the first oligonucleotide by a length of 5-80 bp.

### Claim 7 (depends on 1)

The kit of claim 1, wherein the nanoparticles are conjugated via a thiol group introduced into the terminal of the second oligonucleotide.

### Claim 8 (depends on 1)

The kit of claim 1, wherein the nanoparticles include metal nanoparticles, metal oxide nanoparticles, alloy nanoparticles, and semiconductor nanoparticles.

### Claim 9 (depends on 1)

The kit of claim 1, wherein the DNA nuclease is Mungbean nuclease, Aspergillus nuclease S1, P1 nuclease, or BAL 31 nuclease.

### Claim 10

A method for half-coating of nanoparticles, the method comprising:
(a) self-assembling a first oligonucleotide on a substrate;
(b) hybridizing a second oligonucleotide, complementary to a terminal region of the first oligonucleotide, with the first oligonucleotide;
(c) conjugating nanoparticles to the second oligonucleotide;
(d) coating an exposed surface of the nanoparticles with cucibit [8] Uril (CB), Trp Gly Gly (WGG peptide) and 5,5′Bis(mercaptomethyl)-2,2′-bipyridine; and
(e) treating the product in step (d) with single-stranded DNA nuclease or a double-stranded DNA nuclease to obtain half-coated nanoparticles.

### Claim 11 (depends on 10)

The method of claim 10, wherein the substrate is formed of a metal, a metal oxide, glass, a ceramic, quartz, silicon, a semiconductor, a Si/SiO2 wafer, germanium, gallium arsenide, carbon, carbon nanotubes, a polymer, Sepharose, or agarose.

### Claim 12 (depends on 10)

The method of claim 10, wherein the first oligonucleotide is 10-100 bp (base pair) in length.

### Claim 13 (depends on 10)

The method of claim 10, wherein the terminal region in step (b) is a 3′-terminal region or a 5′-terminal region.

### Claim 14 (depends on 10)

The method of claim 10, wherein the second oligonucleotide in step (b) is 10-100 bp (base pair) in length.

### Claim 15 (depends on 10)

The method of claim 10, wherein the second oligonucleotide in step (b) is hybridized with the terminal region of the first oligonucleotide by a length of 5-80 bp.

### Claim 16 (depends on 10)

The method of claim 10, wherein the nanoparticles in step (b) are conjugated via a thiol group introduced into a terminal of the second oligonucleotide.

### Claim 17 (depends on 10)

The method of claim 10, wherein the nanoparticles in step (c) include metal nanoparticles, metal oxide nanoparticles, alloy nanoparticles, and semiconductor nanoparticles.

### Claim 18 (depends on 10)

The method of claim 10, wherein the DNA nuclease is Mungbean nuclease, Aspergillus nuclease S1, P1 nuclease, or BAL 31 nuclease.