# US 10,864,302 B2 · Adhesion prevention agent comprising injectable thermosensitive wood based-oxidized cellulose nanofiber

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- Number: US 10,864,302 B2
- Document ID: US10864302B2
- Status: Granted
- Kind: B2
- Application number: 15/987,019
- Filed: 2018-05-23
- Published: 2019-01-17
- Granted: 2020-12-15
- Earliest claimed: 2017-07-14
- Assignees: Soonchunhyang University Industry Academy Cooperation Foundation; National Institute of Forest Science
- Applicants: Soonchunhyang University Industry Academy Cooperation Foundation; National Institute of Forest Science
- Inventors: Byong Taek Lee; Sun-Young Lee; Tamanna Sultana; Celine Abueva
- Web page: https://patentgrep.com/patent/US10864302B2

## CPC

- A61L 31/041: {Mixtures of macromolecular compounds}
- A61L 31/042: {Polysaccharides}
- A61L 31/06: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds {(A61L31/041 takes precedence)}
- A61L 31/125: {having a macromolecular matrix}
- A61L 31/14: Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials}
- A61L 31/145: {Hydrogels or hydrocolloids}
- A61L 31/148: {Materials at least partially resorbable by the body}
- A61L 2400/06: Flowable or injectable implant compositions
- A61L 2400/12: Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

The present invention provides a method for preparing an injectable thermosensitive hydrogel for preventing adhesion including mixing methyl cellulose, polyethylene glycol, oxidized cellulose nanofibers and carboxymethyl cellulose. The injectable thermosensitive hydrogel for preventing adhesion is a sol at a low temperature and induces rapid gelation upon in vivo implantation due to thermosensitivity. In addition, the hydrogel can be rapidly changed into physical hydrogel without separate additives or chemical reaction during in vivo condition. In addition, the injectable thermosensitive hydrogel for preventing adhesion exhibits excellent biodegradability and biocompatibility, has no cytotoxicity, inhibits in vitro migration of rat bone marrow mesenchymal stem cells (rBMSCs), and exhibits anti-adhesion efficacy in a rat model of sidewall defect-cecum abrasion in vivo, thus being useful as an effective anti-adhesive agent.

## Claims

### Claim 1

A method for preparing an injectable thermosensitive hydrogel for preventing adhesion comprising: mixing methyl cellulose, polyethylene glycol, oxidized cellulose nanofibers and carboxymethyl cellulose; adding water to the resulting mixture, followed by heating, to prepare a mix solution; and cooling and stirring the solution to prepare an injectable thermosensitive hydrogel for preventing adhesion, wherein the methyl cellulose, the polyethylene glycol, the oxidized cellulose nanofibers and the carboxymethyl cellulose are mixed in a weight ratio of 2:1:0.2:0.8.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the injectable thermosensitive hydrogel shows a sol-gel transition depending on temperature.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the injectable thermosensitive hydrogel is gelled in vivo.

### Claim 4 (depends on 1)

The method according to claim 1, wherein oxidized cellulose nanofibers are derived from wood.

### Claim 5 (depends on 1)

An injectable thermosensitive hydrogel for preventing adhesion prepared by the method according to claim 1.

### Claim 6

An injectable thermosensitive anti-adhesive agent comprising methyl cellulose, polyethylene glycol, oxidized cellulose nanofibers and carboxymethyl cellulose, wherein the methyl cellulose, the polyethylene glycol, the oxidized cellulose nanofibers and the carboxymethyl cellulose are mixed in a weight ratio of 2:1:0.2:0.8.