# US 10,864,237 B2 · Composition for enhanced milk production and feed efficiency and pathogen inhibition > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,864,237 B2 - Document ID: US10864237B2 - Status: Granted - Kind: B2 - Application number: 16/685,727 - Filed: 2019-11-15 - Published: 2020-03-12 - Granted: 2020-12-15 - Earliest claimed: 2010-02-01 - Assignees: Microbios, Inc. - Applicants: Microbios, Inc. - Inventors: Matthew Ryan Garner; Joseph F. Flint - Web page: https://patentgrep.com/patent/US10864237B2 ## CPC - A61K 35/747: Lactobacilli, e.g. L. acidophilus or L. brevis - A23K 10/18: of live microorganisms - A23K 50/10: for ruminants - A23K 50/20: for horses - A23K 50/30: for swines - A23K 50/75: for poultry - A23K 50/80: for aquatic animals, e.g. fish, crustaceans or molluscs - C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) - C12N 1/20: Bacteria; Culture media therefor - C12N 1/205: Bacterial isolates - A23V 2400/181: Salivarius - C12R 2001/23: Lactobacillus acidophilus - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A23K: FODDER - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract Lactobacillus-based probiotic compositions for inclusion in microbial-based products. In one instance, the microbial-based product takes the form of an animal feed additive. When used as a feed additive for animals, especially ruminants, and in particular cattle, the following benefits from the composition are noted: (i) improved milk yield in dairy cattle, (ii) improved milk composition in dairy cattle, (iii) increased weight gain-to-feed intake ratio in beef cattle (feed efficiency), and (vi) improved average daily weight gain. Both Lactobacillus strains MB101 having ATCC Accession Number PTA-121710 and MB102 having ATCC Accession Number PTA-121711 are particularly effective at producing these results. Another marked benefit of the compositions when used as an animal feed additive is inhibition of pathogenic growth including E. coli, Salmonella, Clostridium, Staphylococcus and Streptococcus. ## Claims ### Claim 1 A method of manufacturing live bacterial cells comprising: inoculating a bacterial fermentation medium with live Lactobacillus animalis cells strain MB101 having ATCC Accession Number PTA-121710; and harvesting the live Lactobacillus animalis cells. ### Claim 2 (depends on 1) The method as recited in claim 1, further comprising: inoculating a bacterial fermentation medium with live Propionibacterium cells; and harvesting the live Propionibacterium cells. ### Claim 3 (depends on 2) The method as recited in claim 2, wherein the Propionibacterium cells are one of Propionibacterium freudenreichiim cells and Propionibacterium acidipropionici cells. ### Claim 4 (depends on 1) The method as recited in claim 1, further comprising: harvesting the live Lactobacillus animalis cells strain MB101 having ATCC Accession Number PTA-121710 during at least one logarithmic phase of growth. ### Claim 5 (depends on 1) The method as recited in claim 1, further comprising: harvesting the live Lactobacillus animalis cells strain MB101 having ATCC Accession Number PTA-121710 at least partially during at least one of mid-log phase of growth and late-log phase of growth. ### Claim 6 (depends on 5) The method as recited in claim 5, further comprising: harvesting the live Lactobacillus animalis cells strain MB101 having ATCC Accession Number PTA-121710 during mid-log phase of growth. ### Claim 7 (depends on 5) The method as recited in claim 5, further comprising: harvesting the live Lactobacillus animalis cells strain MB101 having ATCC Accession Number PTA-121710 during late-log phase of growth. ### Claim 8 (depends on 1) The method as recited in claim 1, further comprising: harvesting the live Lactobacillus animalis cells strain MB101 having ATCC Accession Number PTA-121710 between mid-log phase of growth and late-log phase of growth. ### Claim 9 (depends on 1) The method as recited in claim 1, further comprising: harvesting the live Lactobacillus animalis cells strain MB101 having ATCC Accession Number PTA-121710 at least partially during mid-log phase of growth and at least partially during another log phase of growth. ### Claim 10 (depends on 1) The method as recited in claim 1, further comprising: harvesting the live Lactobacillus animalis cells strain MB101 having ATCC Accession Number PTA-121710 when the live cells are substantially spherically shaped. ### Claim 11 A method of manufacturing live bacterial cells comprising: inoculating a bacterial fermentation medium with live Lactobacillus animalis cells strain MB102 having ATCC Accession Number PTA-121711; and harvesting the live Lactobacillus animalis cells. ### Claim 12 (depends on 11) The method as recited in claim 11, further comprising: inoculating a bacterial fermentation medium with live Propionibacterium cells; and harvesting the live Propionibacterium cells. ### Claim 13 (depends on 12) The method as recited in claim 12, wherein the Propionibacterium cells are one of Propionibacterium freudenreichiim cells and Propionibacterium acidipropionici cells. ### Claim 14 (depends on 11) The method as recited in claim 11, further comprising: harvesting the live Lactobacillus animalis cells strain MB102 having ATCC Accession Number PTA-121711 during at least one logarithmic phase of growth. ### Claim 15 (depends on 11) The method as recited in claim 11, further comprising: harvesting the live Lactobacillus animalis cells strain MB102 having ATCC Accession Number PTA-121711 at least partially during at least one of mid-log phase of growth and late-log phase of growth. ### Claim 16 (depends on 15) The method as recited in claim 15, further comprising: harvesting the live Lactobacillus animalis cells strain MB102 having ATCC Accession Number PTA-121711 during mid-log phase of growth. ### Claim 17 (depends on 15) The method as recited in claim 15, further comprising: harvesting the live Lactobacillus animalis cells strain MB102 having ATCC Accession Number PTA-121711 during late-log phase of growth. ### Claim 18 (depends on 11) The method as recited in claim 11, further comprising: harvesting the live Lactobacillus animalis cells strain MB102 having ATCC Accession Number PTA-121711 between mid-log phase of growth and late-log phase of growth. ### Claim 19 (depends on 11) The method as recited in claim 11, further comprising: harvesting the live Lactobacillus animalis cells strain MB102 having ATCC Accession Number PTA-121711 at least partially during mid-log phase of growth and at least partially during another log phase of growth. ### Claim 20 (depends on 11) The method as recited in claim 11, further comprising: harvesting the live Lactobacillus animalis cells strain MB102 having ATCC Accession Number PTA-121711 when the live cells are substantially spherically shaped.