# US 10,863,737 B2 · Culture container, and system and method of transferring a cultured organism between culture containers > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,863,737 B2 - Document ID: US10863737B2 - Status: Granted - Kind: B2 - Application number: 15/692,520 - Filed: 2017-08-31 - Published: 2018-11-22 - Granted: 2020-12-15 - Earliest claimed: 2017-05-22 - Assignees: Drobot Biotechnology Limited Company - Applicants: Drobot Biotechnology Limited Company - Inventors: Chia-Kang Tsao - Web page: https://patentgrep.com/patent/US10863737B2 ## CPC - A01N 1/146: Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving - A01K 67/36: Industrial rearing of insects, e.g. insect farms - A01K 67/362: Containers or crates - A01N 1/142: Apparatus - C12M 21/00: {Bioreactors or fermenters specially adapted for specific uses (digesters for manure A01C3/023; apparatus for PCR B01L7/52; destroying or transforming solid waste B09B3/00; methods for genetic engineering C12N15/00, C12Q1/68; nucleic acid amplification reactions C12Q1/6844)} - C12M 23/08: {Flask, bottle or test tube} - C12M 23/24: {Gas permeable parts} - C12M 23/34: {Internal compartments or partitions} - C12M 23/38: {Caps; Covers; Plugs; Pouring means} - C12M 23/50: {Means for positioning or orientating the apparatus (C12M41/08 takes precedence)} - C12M 33/04: {by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02)} - C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)} - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract A culture container includes a tube having a first and a second opening respectively provided at two opposite ends thereof, and a cover attachable to and removable from the first opening of the tube, the cover including an inlet port for passage of a fluid substance and a receptacle for holding a substance consumable by an organism of interest, the receptacle being enclosed inside the tube and the inlet port communicating with a hollow interior of the tube when the cover is attached to the first opening of the tube. Moreover, a system and a method of transferring a cultured organism of interest include switching the covers between two culture containers. ## Claims ### Claim 1 A transfer system comprising: a first conveyor system for transporting at least a first culture container along a first path, wherein the first culture container includes a first tube having two openings at two opposite ends thereof and a removable first cover attached to one of the two openings of the first tube, the first cover including a first receptacle for holding a substance consumable by an organism of interest, the first receptacle being enclosed inside the first tube when the first cover is attached to the first tube; a second conveyor system for transporting at least a second culture container along a second path, wherein the second culture container includes a second tube having two openings at two opposite ends thereof and a removable second cover attached to one of the two openings of the second tube, the second cover including a second receptacle for holding a substance consumable by an organism of interest, the second receptacle being enclosed inside the second tube when the second cover is attached to the second tube; and an exchanging unit disposed between the first and second conveyor systems, the exchanging unit being operable to interchange the first and second covers between the first and second culture containers so that the first cover is attached to the second tube and the second cover is attached to the first tube. ### Claim 2 (depends on 1) The transfer system according to claim 1, wherein the exchanging unit is operable to: respectively separate the first and second covers from the first and second tubes; switch the first and second covers with respect to the first and second tubes; and respectively attach the first cover to the second tube and the second cover to the first tube. ### Claim 3 (depends on 2) The transfer system according to claim 2, wherein the exchanging unit switches the first and second covers with respect to the first and second tubes by moving the first and second covers while keeping the first and second tubes stationary, or by moving the first and second tubes while keeping the first and second covers stationary. ### Claim 4 (depends on 2) The transfer system according to claim 2, wherein the exchanging unit includes a rotary part operable to switch the first and second covers with respect to the first and second tubes. ### Claim 5 (depends on 4) The transfer system according to claim 4, wherein the exchanging unit further includes two arms that are connected with the rotary part at two diametrically opposite positions relative to a pivot axis of the rotary part, each of the two arms being operable to separate or attach one cover with respect to one tube, the rotary part and the two arms being rotatable to move the first and second covers while the first and second tubes remain stationary. ### Claim 6 (depends on 5) The transfer system according to claim 5, wherein each of the two arms is configured to hold one cover at a catch portion provided on the cover. ### Claim 7 (depends on 4) The transfer system according to claim 4, wherein the exchanging unit further includes two arms extending above the rotary part, each of the two arms being operable to separate or attach one cover with respect to one tube, and the rotary part being rotatable to move the first and second tubes while the first and second covers are kept stationary by the two arms. ### Claim 8 (depends on 1) The transfer system according to claim 1, further comprising an anesthetization unit disposed adjacent to the first conveyor system and upstream of the exchanging unit, the anesthetization unit being operable to anesthetize an organism of interest enclosed inside the first culture container. ### Claim 9 (depends on 8) The transfer system according to claim 8, wherein the anesthetization unit is operable to deliver an anesthetic substance into the first culture container through the first cover thereof for anesthetizing an organism of interest enclosed inside the first culture container. ### Claim 10 (depends on 9) The transfer system according to claim 9, wherein the first cover attached to the first tube has a sealing film for preventing fluid passage through the first inlet port into the hollow interior of the first tube, and the anesthetization unit is further operable to pierce the sealing film before delivering the anesthetic substance into the first culture container. ### Claim 11 (depends on 1) The transfer system according to claim 1, further comprising a first sealing unit operable to bond a first sealing film on the second cover attached to the first tube, and a second sealing unit operable to bond a second sealing film on the first cover attached to the second tube. ### Claim 12 (depends on 1) The transfer system according to claim 1, further comprising a reading unit disposed adjacent to the second path, and a printing unit disposed adjacent to the first path, the reading unit being operable to read a code on the first cover attached to the second tube, and the printing unit being operable to print an identification code on the second cover attached to the first tube according to the code read on the first cover. ### Claim 13 (depends on 1) The transfer system according to claim 1, wherein the first conveyor system includes a rotary platform, a container supplying part for delivering the first culture container comprised of the first tube and the first cover to the rotary platform, and a container discharging part, the rotary platform including at least one slot for receiving the first tube, the rotary platform being operable to convey the first tube received in the slot from the container supplying part through the exchanging unit to the container discharging part. ### Claim 14 (depends on 13) The transfer system according to claim 13, wherein any of the container supplying part and the container discharging part includes a ramp or a conveying belt. ### Claim 15 (depends on 1) The transfer system according to claim 1, wherein the second conveyor system includes a second rotary platform, a second container supplying part for delivering the second culture container comprised of the second tube and the second cover to the second rotary platform, and a second container discharging part, the second rotary platform including at least one second slot for receiving the second tube, the second rotary platform being operable to convey the second tube received in the second slot from the second container supplying part through the exchanging unit to the second container discharging part. ### Claim 16 (depends on 1) The transfer system according to claim 1, wherein the first and second conveyor systems are operable to respectively carry the first and second culture containers toward the exchanging unit with the first and second covers respectively on top of the first and second tubes. ### Claim 17 (depends on 1) The transfer system according to claim 1, further comprising a pressurized chamber, the exchanging unit being disposed inside the pressurized chamber, and the first and second conveyor systems are respectively operable to convey the first and second culture containers into the pressurized chamber.