# US 10,863,736 B2 · Aliquoting and freezing storage device

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- Number: US 10,863,736 B2
- Document ID: US10863736B2
- Status: Granted
- Kind: B2
- Application number: 15/315,508
- Filed: 2016-07-27
- Published: 2017-07-06
- Granted: 2020-12-15
- Earliest claimed: 2013-11-20
- Applicants: Xiaomin Cai
- Inventors: Xiaomin Cai
- Web page: https://patentgrep.com/patent/US10863736B2

## CPC

- A01N 1/146: Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
- B01L 9/065: {specially adapted for capillary tubes}
- G01N 1/42: Low-temperature sample treatment, e.g. cryofixation
- B01L 2200/025: Align devices or objects to ensure defined positions relative to each other
- B01L 2300/0609: Holders integrated in container to position an object
- B01L 2300/0809: rectangular shaped
- B01L 2300/0838: Capillaries
- B01L 2300/0841: Drums
- B01L 2300/16: Surface properties and coatings
- B01L 2300/1894: Cooling means; Cryo cooling
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

An aliquoting and freezing storage device comprises a test tube and at least one capillary tube. The capillary tube is inserted into a test tube, and the capillary tube is provided with an inner long hole which penetrates throughly. The device utilizes the capillarity to quickly and easily store biological samples in the capillary tubes. When needed, the operator only needs to remove one or several of the tubes, without affecting the remaining biological samples, so as to avoid repeated freezing and thawing of biological samples.

## Claims

### Claim 1

An aliquoting-freezing storage device comprising a test tube (300), a capillary supporter (200), and at least one capillary tube (100) having an upper and a lower end,
wherein the capillary tube (100) contains a frozen sample in storage,
wherein the capillary tube (100) is inserted in the test tube (300), and the capillary tube (100) has a long hole through the upper and the lower end,
wherein the test tube (300) also contains the frozen sample that is outside the capillary tube (100) and at the bottom of the test tube (300),
wherein the capillary tube (100) is threaded through the capillary supporter (200); the capillary supporter's (200) outer-diameter is matched with the inner-diameter of the test tube (300); the capillary support (200) is provided with the positioning holes (211) which are uniformly distributed in the circumferential direction, and
wherein the capillary supporter (200) comprises a support plate (210) and legs (220), the legs (220) are provided on the bottom outer edge of the support plate (210); the upper surface of the support plate (210) is circular and has a main handle (212) which is convex at the center of the upper surface; and the positioning holes (211) are annularly arrayed around the main handle (212) on the support plate (210).

### Claim 2 (depends on 1)

The aliquoting-freezing storage device according to claim 1, wherein the capillary tube (100) comprises a co-axial capillary tube handle (110) and a capillary body (120); and the capillary tube handle is fixedly disposed at the upper end of the capillary body (120).

### Claim 3 (depends on 1)

The aliquoting-freezing storage device according to claim 1, characterized in that the outer wall of the capillary tube (100) is coated with a non-sticking film.

### Claim 4 (depends on 1)

The aliquoting-freezing storage device according to claim 1, characterized in that the legs (220) are in strip foul) and have at least two strips, and are fixedly disposed at the bottom outer edge of the support plate (210); and the support legs (220) are circumferentially arranged around the center of the support plate (210).

### Claim 5 (depends on 1)

The aliquoting-freezing storage device according to claim 1, characterized in that the legs (220) are a hollow cylinder; the bottom edge of the support plate (210) is concavely shaped to conform to the legs (220); and the legs (220) are inserted into a groove of the support plate (210).

### Claim 6

An aliquoting-freezing storage device comprising a test tube (300), a capillary supporter (200), and at least one capillary tube (100) having an upper and a lower end,
wherein the capillary tube (100) contains a frozen sample in storage,
wherein the capillary tube (100) is inserted in the test tube (300), and the capillary tube (100) has a long hole through the upper and the lower end,
wherein the test tube (300) also contains the frozen sample that is outside the capillary tube (100) and at the bottom of the test tube (300),
wherein the capillary tube (100) is threaded through the capillary supporter (200); the capillary supporter's (200) outer-diameter is matched with the inner-diameter of the test tube (300); the capillary support (200) is provided with positioning holes (211) which are uniformly distributed in the circumferential direction, and
wherein the capillary holder (200) comprises a support plate (210) and legs (220); the legs (220) each has a strip shape and has at least two strips, and are fixedly arranged on the top outer edge of the support plate (210); the support plate (210) is circular, and the central of the upper surface is provided with an outwardly protruding main handle (212), the positioning holes (211) are arranged at the top of the support plate (210); the legs (220) are circumferentially arranged around the center of the support plate (210); the top of the legs (220) are provided with an outward positioning hook (221); and the outer diameter of the positioning hook (221) is larger than the inner diameter of the test tube (300).
