Arrays of integrated analytical devices
AssigneePacific Biosciences of California, Inc.
InventorsAnnette Grot, Ravi Saxena, Paul Lundquist
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Abstract
Arrays of integrated analytical devices and their methods for production are provided. The arrays are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices allow the highly sensitive discrimination of optical signals using features such as spectra, amplitude, and time resolution, or combinations thereof. The devices include an integrated diffractive beam shaping element that provides for the spatial separation of light emitted from the optical reactions.
Claims30
An array of integrated analytical devices, each device comprising:
- a nanoscale emission volume;
- a detector layer optically coupled to the nanoscale emission volume;
- a laser rejection filter layer disposed between the nanoscale emission volume and the detector layer;
- and
- an excitation source optically coupled to the nanoscale emission volume;
- wherein light is emitted from the nanoscale emission volume by a plurality of emitters within the emission volume;
- wherein the detector layer comprises a plurality of sensing regions;
- and
- wherein the laser rejection filter layer comprises a multilayer filter element comprising a dielectric interference filter layer and a low index total internal reflectance layer or a hybrid laser rejection filter element comprising an absorption dye layer and a dielectric interference stack layer.
Dependencies are inferred from the claim text.