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US 10,858,692 B2Granted
Methods for making nucleotide probes for sequencing and synthesis
OwnerGeorge M. Church, Kun Zhang +1inventors
InventorsGeorge M. Church, Kun Zhang, Joseph Chou
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Abstract
Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.
Claims8
A method for processing a probe for analyzing a genomic deoxyribonucleic acid (DNA) region of a genomic DNA sample, the method comprising:
- (a) providing a plurality of probes, wherein a probe of the plurality of probes has two regions of degenerate homology to target genomic DNA at the ends of the probe, two primer regions common to other probes of the plurality of probes, and a bar code specific for the genomic DNA region of the genomic DNA sample;
- (b) hybridizing the probe to the genomic DNA region such that the probe is hybridized to the genomic DNA region in a circular manner;
- (c) ligating portions of the probe to one another to produce a closed circular molecule;
- (d) separating the closed circular molecule from the genomic DNA region;
- and
- (e) amplifying the closed circular molecule, in an emulsion polymerase chain reaction (ePCR) or polony amplification reaction, to produce amplified DNA, wherein the closed circular molecule is not cleaved prior to the amplifying.
3 more elements
Dependencies are inferred from the claim text.