Method for producing objective substance
AssigneeAjinomoto Co., Inc.
InventorsChristine Roche, Benjamin Mijts, Peter Kelly, Matthew Davis, Sayaka Asari, Keita Fukui, Miku Toyazaki
Life of this patent
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Abstract
A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so as to have a specific feature, such as a reduced activity of AICAR formyltransferase/IMP cyclohydrolase, an increased activity of 3-PGDH, and/or a reduced activity of L-serine deaminase.
Claims45
A method for producing an objective substance, the method comprising:
- producing the objective substance by using a microorganism having an ability to produce the objective substance;
- wherein the microorganism has a feature selected from the group consisting of
- (A) a first feature selected from the group consisting of
- (A-i) the microorganism has been modified so that the activity of AICAR formyltransferase/IMP cyclohydrolase is reduced as compared with a non-modified microorganism,
- (A-ii) the microorganism has been modified so that a gene encoding AICAR formyltransferase/IMP cyclohydrolase has a mutation that improves the ability of the microorganism to produce the objective substance, and
- (A-iii) combinations thereof;
- (B) a second feature selected from the group consisting of
- (B-i) the microorganism has been modified so that the activity of D-3-phosphoglycerate dehydrogenase is increased as compared with a non-modified microorganism,
- (B-ii) the microorganism has been modified so as to have a gene encoding D-3-phosphoglycerate dehydrogenase resistant to feedback inhibition by L-serine, and
- (B-iii) combinations thereof;
- (C) the microorganism has been modified so that the activity of L-serine deaminase is reduced as compared with a non-modified microorganism;
- and
- (D) combinations thereof;
- wherein the objective substance is a metabolite selected from the group consisting of vanillin, vanillic acid, melatonin, ergothioneine, mugineic acid, ferulic acid, polyamine, guaiacol, 4-vinylguaiacol, 4-ethylguaiacol, creatine, and combinations thereof.
Dependencies are inferred from the claim text.