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AssigneeThe Government of the United States of America, as represented by the Secretary of Homeland Security
InventorsMichael Puckette, Max V. Rasmussen
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This application is directed generally to foot-and-mouth disease virus (FMDV) 3C proteases that have been modified by mutating a polynucleotide sequence coding for the FMDV 3C protease. The modified FMDV proteases exhibit proteolytic activity on FMDV P1 precursor protein and exhibit a reduction in one or more toxic or inhibitory properties associated with an unmodified FMDV 3C protease on a host cell used to recombinantly produce it. Vectors carrying polynucleotides encoding modified FMDV 3C protease sequences can induce production of FMDV virus-like particles in a host cell when expressed in the host cell. The modified FMDV 3C proteases can generally be used to produce immunogenic FMDV preparations capable of inducing an immune response against FMDV.
A method for producing foot-and-mouth disease virus (FMDV) viral proteins, comprising culturing a host cell in a suitable medium, the host cell comprising and expressing:
A foot-and-mouth disease virus (FMDV) viral protein selected from the group consisting of VP0, VP1, VP2, VP3, VP4 and FMDV virus-like particles (VLPs), the viral protein produced by a method comprising:
A method for producing foot-and-mouth disease virus (FMDV) viral proteins, comprising culturing a host cell in a suitable medium, the host cell comprising and expressing:
Dependencies are inferred from the claim text.