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US 10,858,622 B2Granted
Heatseal sampling
AssigneeGlobal Life Sciences Solutions USA LLC
InventorsManoj Ramakrishna, Haresh Digambar Patil, Sebastian John
Life of this patent
Estimating the term…
Abstract
The present invention provides a device and method for sampling from a cell culture bioreactor. The invention is useful where it is required to determine the cell count, provide a sample for off-line analyses or remove a sample while at the same time retain sterility of the sample for quality control (QC) assessment. The device of the invention allows for facile aseptic sampling at one or more instances in time during a cell expansion in a bioreactor.
Claims22
A device for taking a sample from a liquid cell culture in a bioreactor wherein said device comprises:
- (i) a hollow body comprising outer walls that define a fluid path therethrough wherein said outer walls are at least partly comprised of a sealable material, wherein said fluid path begins at an upstream end of said hollow body, through said hollow body, and ends at a downstream end of said hollow body, and wherein said upstream and downstream ends are adapted to fluidly connect said fluid path with an interior of said bioreactor, wherein the liquid cell culture flows from the interior of said bioreactor into the fluid path via the upstream end and flows back to the interior of said bioreactor via the downstream end;
- and,
- (ii) a sealer which is adapted to seal a perimeter of a section of said sealable material without blocking said fluid path during the liquid cell culture passing through the fluid path, wherein said sealer is moveable between different portions of said outer walls.
Dependencies are inferred from the claim text.