Back to results
US 10,858,385 B2Granted
Methods and compositions for nucleoside triphosphate and ribonucleic acid production
AssigneeGreenLight Biosciences, Inc.
InventorsDrew S. Cunningham, Daniel MacEachran, James Robbins Abshire, Himanshu Dhamankar, Ifeyinwa Iwuchukwu, Mehak Gupta, Matthew Eduardo Moura, Naveen Sudharsan, Nicholas Skizim, Rachit Jain, Karthikeyan Ramachandriya
Life of this patent
Estimating the term…
Abstract
Provided herein, in some embodiments, are methods and composition for the production of nucleoside triphosphates and ribonucleic acids.
Claims30
A method for producing ribonucleic acid (RNA) comprising:
- (a) incubating (i) cellular RNA, wherein the cellular RNA comprises ribosomal RNA, messenger RNA, and/or transfer RNA, and (ii) a ribonuclease to produce 5′ nucleoside monophosphates (NMPs);
- (b) eliminating the ribonuclease;
- and
- (c) incubating the 5′ NMPs with polyphosphate kinase (PPK), polyphosphate, deoxyribonucleic acid (DNA) template encoding a RNA of interest, and RNA polymerase to produce the RNA of interest.
1 more element
A method for producing ribonucleic acid (RNA) comprising:
- (a) incubating (i) cellular RNA and (ii) a ribonuclease to produce 5′ nucleoside monophosphates (NMPs);
- (b) eliminating the ribonuclease;
- and
- (c) incubating the 5′ NMPs with at least one NMP kinase, at least one NDP kinase, at least one polyphosphate kinase (PPK), polyphosphate, deoxyribonucleic acid (DNA) template encoding a RNA of interest, and RNA polymerase to produce the RNA of interest.
1 more element
A method for producing ribonucleic acid (RNA) comprising:
- (a) incubating (i) cellular RNA and (ii) Nuclease P1 to produce 5′ nucleoside monophosphates (NMPs);
- (b) eliminating the Nuclease P1;
- and
- (c) incubating the 5′ NMPs with at least one NMP kinase, at least one NDP kinase, a Class III PPK2 enzyme from Deinococcus geothermalis (SEQ ID NO:
- 1), polyphosphate, deoxyribonucleic acid (DNA) template encoding a RNA of interest, and RNA polymerase to produce the RNA of interest.
2 more elements
A method for producing ribonucleic acid (RNA) comprising:
- (a) incubating (i) cellular RNA and (ii) a ribonuclease to produce 5′ nucleoside monophosphates (NMPs);
- (b) eliminating the ribonuclease, wherein the ribonuclease is eliminated via temperature, pH, salt, detergent, alcohol, chemical inhibitors, separation, precipitation, filtration, capture, and/or chromatography;
- and
- (c) incubating the 5′ NMPs with polyphosphate kinase (PPK), polyphosphate, deoxyribonucleic acid (DNA) template encoding a RNA of interest, and RNA polymerase to produce the RNA of interest.
1 more element
Dependencies are inferred from the claim text.