Enzymatically responsive magnetic particles and their use
AssigneeBrandeis University
InventorsXuewen Du, Jie Zhou, Bing Xu
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Abstract
The invention relates to an enzymatically responsive product that includes an amino acid residue conjugated to a magnetic particle, wherein the amino acid residue is phosphorylated or sulfated or comprises an ester-moiety linked via peptide bond. Compositions containing the enzymatically responsive product, and the use thereof for separating distinct types of mammalian cells (e.g., cancer cells from normal cells), for treating a cancerous condition, and imaging cancer cells are also disclosed.
Claims15
A product comprising a single amino acid residue conjugated to a magnetic particle, wherein the single amino acid residue is not present in a polypeptide or protein, and wherein the single amino acid is selected from the group consisting of phosphoserine, sulfoserine, phosphothreonine, sulfothreonine, phosphohistidine, sulfohydroxyproline, phosphotyrosine, and sulfotyrosine.
A product comprising a single, phosphorylated amino acid residue (pAA) conjugated to a magnetic particle—wherein the magnetic particle comprises a polymer conjugated to the single, phosphorylated amino acid residue (pAA), where n is any integer
- wherein the single, phosphorylated amino acid residue (pAA) is selected from phosphoserine, phosphothreonine, phosphohistidine, and phosphotyrosine.
A product comprising a polypeptide that includes from 2 to 35 amino acid residues, one of the amino acid residues being conjugated to a magnetic particle and one of the amino acid residues being phosphorylated or sulfated or comprising an ester-moiety linked via peptide bond, wherein the polypeptide further comprises a metalloprotease (MMP) cleavage site and an amino acid sequence selected from the group consisting of:
- SEQ.
- Polypeptide
- ID. NO:
- X-Gly-Pro-Gln-Gly↓Leu-Ala-Gly-Gln
- 1
- X-Ala-Pro-Ala-Ala↓Leu-Val-Gly-Ala
- 2
- X-Ala-Pro-Ala-Gly↓Leu-Lys-Ala-Pro
- 3
- X-Glu-Pro-Ala-Ser↓Leu-Arg-Ala-Gly
- 4
- X-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Arg
- 5
- X-Gly-Pro-Ala-Gly↓Leu-Arg-Gly-Pro
- 6
- X-Gly-Pro-Ala-Gly↓Leu-Gly-Ala-Ala
- 7
- X-Gly-Pro-Lys-Gly↓Leu-Arg-Gly-Gly
- 8
- X-Leu-Pro-Leu-Gly↓Leu-Val-Thr-Glu
- 9
- wherein X is any aromatic group, aromatic amino acid residue, or hydrophobic amino acid;
- and ↓ is a MMP cleavage site.
Dependencies are inferred from the claim text.