Gas-phase purification for accurate isobaric tag-based quantification

A mass spectrometer system for analyzing an isobarically labeled analyte, the system comprising:

• an ion source for generating ions from the isobarically labeled analyte in a mixture with one or more molecules able to generate contaminant ions during mass spectrometry;
• first ion separation optics in communication with the ion source for separating ions according to their mass-to-charge ratios;
• ion reaction optics in communication with the first ion separation optics for generating mass-to-charge-manipulated ions;
• ion fragmentation optics in communication with the first ion separation optics for generating product ions;
• second ion separation optics in communication with the ion fragmentation optics for separating ions according to their mass-to-charge ratios;
• a first ion detector in communication with the second ion separation optics for detecting ions separated according to their mass-to-charge ratios;
• a controller operably connected to the first and second ion separation optics, the ion reaction optics, the first ion detector, and the ion fragmentation optics;
• wherein the controller controls the ion optics and detector so as to:
• (a) generate a first distribution of precursor ions from the mixture during MS1 stage ionization;
• (b) identify a target range of mass-to-charge ratios of the first distribution of precursor ions, wherein said target range includes mass-to-charge ratios of precursor ions generated from the isobarically labeled analyte as well as contaminant ions;
• (c) via the first separation optics, isolate precursor ions from the first distribution of precursor ions, where the isolated precursor ions have mass-to-charge ratios within the identified target range, wherein precursor ions outside of the identified target range are removed from communication with the ion reaction optics;
• (d) reacting the isolated precursor ions within the identified target range to selectively change the mass-to-charge ratios of the isolated precursor ions by a known amount, thereby generating a distribution of mass-to-charge-manipulated precursor ions, wherein precursor ions outside of the identified target range are not reacted;
• (e) identify a desired range of mass-to-charge ratios of the distribution of mass-to-charge-manipulated precursor ions, wherein the desired range includes mass-to-charge ratios of precursor ions generated from the isobarically labeled analyte and manipulated by a known amount as described in step (d), and wherein the desired range does not include mass-to-charge ratios of contaminant ions manipulated as described in step (d);
• (f) via the second separation optics, separate mass-to-charge-manipulated precursor ions having a mass-to-charge ratio within the desired range from mass-to-charge-manipulated contaminant ions having a mass-to-charge ratio outside of the desired range, thereby generating isolated mass-to-charge-manipulated precursor ions having a mass-to-charge ratio within the desired range, wherein the mass-to-charge-manipulated contaminant ions are removed from communication with the ion fragmentation optics;
• (g) fragment ions corresponding to the isolated mass-to-charge-manipulated precursor ions during MS2 fragmentation, thereby generating first product ions, wherein the mass-to-charge-manipulated contaminant ions are not fragmented;
• and
• (h) measure the mass-to-charge ratios of the first product ions, thereby generating first product ion mass spectrometry data.