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US 10,852,302 B2Granted
Method for evaluation of function of phagocyte
AssigneeMochida Pharmaceutical Co., Ltd.
InventorKatsuki Naito
Life of this patent
Estimating the term…
Abstract
A novel convenient method for evaluating the function of a phagocyte is provided. The method assays sCD14-ST, which is a humoral factor specifically produced in phagocytosis by the phagocyte and which is stable enough for use in an assay. Also provided is a method for detecting diseases associated with the phagocytosis by the phagocyte.
Claims4
A method for detecting a disease associated with phagocytosis by a phagocyte comprising the steps of:
- obtaining a specimen from a subject, wherein said specimen is at least one body fluid selected from the group consisting of interstitial fluid, lymph, synovial fluid, milk, cerebrospinal fluid, pus, saliva, lacrimal fluid, mucus, nasal discharge, sputum, urine, ascites, amniotic fluid, seminal fluid and lavage fluids obtained after washing naval cavity, bronchus, lung, skin, peritoneal cavity and various organs;
- adding to the specimen a substance which increases phagocytic activity and which is engulfed by phagocytes in the specimen and which induces phagocytes in the specimen to produce an amount of sCD14-ST before measuring a total amount of sCD14-ST in the specimen;
- measuring the total amount of sCD14-ST, including the amount sCD14-ST which is produced by phagocytes after the phagocytosis of the substance;
- comparing the measured value with a standard value;
- determining whether the amount of the sCD14-ST in the specimen is higher than the standard value;
- and
- detecting a disease associated with phagocytosis by a phagocyte in the subject when the measured value is higher than the standard value,
- wherein the sCD14-ST is measured by a sandwich immunoassay system comprising:
- (a) an antibody binding to a peptide comprising 16 amino acids spanning from 53rd to 68th amino acids of human CD14 of SEQ ID NO:
- 1, and
- (b) an antibody binding to a peptide comprising an amino acid sequence at position 17 to 26 of SEQ ID NO:
- 1.
9 more elements
A method for detecting a rheumatoid arthritis, which comprises the steps of
- obtaining a specimen from a subject, wherein said specimen is a synovial fluid:
- adding to the specimen a substance which increases phagocytic activity and which is engulfed by phagocytes in the specimen before measuring the amount of sCD14-ST in the specimen;
- measuring the amount of sCD14-ST which is produced by phagocytes after the phagocytosis of the substance;
- comparing the measured value with a standard value;
- determining whether the amount of the sCD14-ST in the specimen is higher than the standard value;
- and
- detecting rheumatoid arthritis in the subject when a measured value is higher than the standard value, and
- wherein the sCD14-ST is measured by a sandwich immunoassay system comprising:
- (a) an antibody binding to a peptide comprising 16 amino acids spanning from 53rd to 68th amino acids of human CD14 of SEQ ID NO:1, and
- (b) an antibody binding to a peptide comprising an amino acid sequence at position 17 to 26 of SEQ ID NO:
- 1.
8 more elements
A method for detecting mastitis, which comprises the steps of:
- obtaining a specimen from a subject, wherein said specimen comprises milk:
- adding to the specimen a substance which increases phagocytic activity and which is engulfed by phagocytes in the specimen before measuring the amount of sCD14-ST in the specimen;
- measuring the amount of sCD14-ST which is produced by phagocytes after the phagocytosis of the substance;
- comparing the measured value with a standard value;
- determining whether the amount of the sCD14-ST in the specimen is higher than the standard value;
- and
- detecting mastitis in the subject when a measured value is higher than the standard value, and
- wherein the sCD14-ST is measured by a sandwich immunoassay system comprising:
- (a) an antibody binding to a peptide comprising 16 amino acids spanning from 53rd to 68th amino acids of human CD14 of SEQ ID NO:1, and
- (b) an antibody binding to a peptide comprising an amino acid sequence at position 17 to 26 of SEQ ID NO:
- 1.
8 more elements
Dependencies are inferred from the claim text.