Micro-sampling for cell, tissue, and micro-organism monitoring

An apparatus for spatial and temporal, monitoring, analysis, and chemical mapping of a living cell aggregate, comprising:

• a culture volume containing the living cell aggregate, the living cell aggregate comprising one or more aggregate regions,
• an addressable and controllable input barrier comprising a plurality of apertures in fluid communication with the culture volume and configured to provide fluidic control of input media into the culture volume, such that selective and differential input media is delivered to at least one of the one or more aggregate regions,
• an addressable and controllable output barrier comprising a plurality of apertures, each of the plurality of apertures of the output barrier corresponding to at least one of the plurality of apertures of the input barrier proximate a same region of the living cell aggregate, wherein each of the plurality of apertures of the output barrier is configured to receive extracellular fluidic output comprising a product of biological activity resulting from the living cell aggregate responding to the input media provided to the corresponding at least one of the plurality of apertures of the input barrier, and
• an analyzer in fluid communication with the output barrier and configured to analyze the extracellular fluidic output
• from each of the plurality of apertures of the output barrier individually, wherein the analyzer is configured to generate a chemical map of the living cell aggregate based on individual analysis results performed on the extracellular fluidic output from each of the plurality of apertures of the output barrier.