Dip-stick western blot
AssigneeBio-Rad Laboratories, Inc.
InventorWilliam Strong
Life of this patent
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Abstract
Methods, kits, and systems are provided for separating, immobilizing, and/or detecting analytes of one or more samples using dipsticks. A ‘dipstick’ is an object that can be embedded and subsequently removed from a separation medium, and to which analytes can be immobilized while the object is embedded in the separation medium. Examples of separation media include an electrophoresis gel of any format and a stationary phase for column chromatography. Embodiments of the present methods include applying a sample to a separation medium; separating analytes of the sample in the separation medium along a separation axis; immobilizing the analytes on a dipstick embedded in the separation medium; removing the dipstick from the separation medium; and detecting the analytes immobilized on the removed dipstick.
Claims9
A method of separating and detecting analytes of a sample, the method comprising:
- applying a sample to a fluid separation medium, wherein the fluid separation medium comprises polyacrylamide or dextran;
- separating analytes of the sample in the separation medium to generate a distribution of the analytes along a separation axis;
- immobilizing the analytes on a dipstick embedded in part of the separation medium in which analytes become distributed such that the dipstick or an elongated portion thereof is aligned parallel to the separation axis, wherein distribution of analytes immobilized on the dipstick preserves the distribution of analytes as occurred in the separation medium, wherein the dipstick is embedded before the separating;
- removing the dipstick from the separation medium;
- and
- detecting the analytes immobilized on the removed dipstick,
- wherein separating analytes of the sample comprises performing electrophoresis, electroosmosis, or isoelectric focusing.
Dependencies are inferred from the claim text.