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US 10,852,272 B2Granted
Devices and methods using pore size modulation for detecting analytes in a fluid sample
AssigneeThe Regents of the University of California
InventorsAmy E. Herr, Todd A. Duncombe
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Abstract
Provided are devices that include a polymeric separation medium configured to immobilize one or more constituents of interest in the polymeric separation medium and have an increased pore size upon application of an applied stimulus. Systems including the devices, as well as methods of using the devices, are also provided. Embodiments of the present disclosure find use in a variety of different applications, including detecting whether an analyte is present in a fluid sample.
Claims24
A device comprising:
- a polymeric separation medium that immobilizes one or more constituents of interest in the polymeric separation medium and has an increased pore size upon application of an applied stimulus,
- wherein the polymeric separation medium comprises a non-labile crosslinker configured to crosslink the polymeric separation medium, and a labile crosslinker configured to crosslink the polymeric separation medium and de-crosslink upon application of the applied stimulus, wherein the labile crosslinker comprises N,N′-[(1-methylethylidene)bis(oxy-2,1-ethanediyl)]diacrylamide (DK),
- wherein the polymeric separation medium comprises functional groups that covalently bond to one or more of the constituents of interest in the polymeric separation medium.
A method of detecting an analyte in a fluid sample, the method comprising:
- introducing a fluid sample into a device comprising a polymeric separation medium that immobilizes one or more constituents of interest in the polymeric separation medium and has an increased pore size upon application of an applied stimulus, wherein the polymeric separation medium comprises a non-labile crosslinker configured to crosslink the polymeric separation medium, and a labile crosslinker configured to crosslink the polymeric separation medium and de-crosslink upon application of the applied stimulus, wherein the labile crosslinker comprises N,N′-[(1-methylethylidene)bis(oxy-2,1-ethanediyl)]diacrylamide (DK);
- applying an electric field across the polymeric separation medium in a manner sufficient to separate components of the fluid sample in the polymeric separation medium to provide a separated sample;
- contacting the polymeric separation medium with the applied stimulus, thereby causing the labile crosslinker to decrosslink and the separation medium to have an increased pore size;
- and
- detecting the analyte in the separated sample.
2 more elements
Dependencies are inferred from the claim text.