Microarray, imaging system and method for microarray imaging

An imaging system comprising:

• an exciting light source configured to emit light;
• a light guide connected with the exciting light source to guide the light;
• a microarray disposed on a transmission path of the light, the microarray comprising a plurality of detection areas and at least one mark, wherein the at least one mark is configured for at least one of image focusing, positioning and splicing as well as comprises a plurality of grid dots distributed in an area, the area comprises a plurality of zones arranged in an array, each of the plurality of the grid dots is disposed in one of the plurality of the zones, a number of the plurality of the grid dots is less than a number of the plurality of the zones, and the plurality of the grid dots form a two-dimensional pattern, wherein the microarray is configured to switch to a first light mode and perform coarse focusing on the at least one mark, perform fine focusing on the at least one mark, and switch to a second light mode;
• and an image capturing device configured to capture an image of the microarray based on the at least one mark the fine focusing result.

A method for microarray imaging, comprising:

• providing a microarray comprising a plurality of detection areas and a plurality of marks, wherein each of the plurality of the marks is configured for at least one of image focusing, positioning and splicing, each of the plurality of the marks comprises a plurality of grid dots distributed in an area, the area comprises a plurality of zones arranged in an array, each of the plurality of the grid dots is disposed in one of the plurality of the zones, a number of the plurality of the grid dots is less than a number of the plurality of the zones, and the plurality of the grid dots form a two-dimensional pattern;
• switching to a first light mode and performing coarse focusing on the plurality of the marks;
• performing fine focusing on the plurality of the marks;
• and switching to a second light mode and capturing a plurality of images of the microarray based on the fine focusing result.