Reagents and methods for analysis of HIV

A composition that is an amplification reaction mixture, the composition comprising:

• an HIV nucleic acid molecule;
• a pair of oligonucleotide primers comprising a forward oligonucleotide primer and a reverse oligonucleotide primer that, upon hybridization to the HIV nucleic acid molecule, flank an amplicon sequence, wherein the forward oligonucleotide primer comprises a nucleic acid sequence according to one of SEQ ID NOs:
• 23 and 35-39 and the reverse oligonucleotide primer comprises a nucleic acid sequence complementary to a nucleic acid sequence according to one of SEQ ID NOs:
• 40 and 52-55;
• at least one probe, wherein the at least one probe comprises a labeled oligonucleotide probe comprising a fluorescent moiety;
• and
• an amplicon molecule having a sequence derived from a portion of the HIV nucleic acid molecule sequence, and the sequence of the forward oligonucleotide primer or the sequence of the reverse oligonucleotide primer.

A kit comprising:

• a pair of oligonucleotide primers comprising a forward oligonucleotide primer and a reverse oligonucleotide primer that, upon hybridization to an HIV nucleic acid molecule, flank an amplicon sequence, wherein the forward oligonucleotide primer comprises a nucleic acid sequence according to one of SEQ ID NOs:
• 23 and 35-39 and the reverse oligonucleotide primer comprises a nucleic acid sequence complementary to a nucleic acid sequence according to one of SEQ ID NOs:
• 40 and 52-55;
• and
• at least one probe, wherein the at least one probe comprises a labeled oligonucleotide probe comprising a fluorescent moiety.

A composition comprising:

• a pair of oligonucleotide primers comprising a forward oligonucleotide primer and a reverse oligonucleotide primer that, upon hybridization to the HIV nucleic acid molecule, flank an amplicon sequence, wherein the forward oligonucleotide primer comprises a nucleic acid sequence according to one of SEQ ID NOs:
• 23 and 35-39 and the reverse oligonucleotide primer comprises a nucleic acid sequence complementary to a nucleic acid sequence according to one of SEQ ID NOs:
• 40 and 52-55;
• at least one probe, wherein the at least one probe comprises a labeled oligonucleotide probe comprising a fluorescent moiety;
• and
• a buffer.