Methods of determining an immune response score

A method for treating a subject with an anticancer immunotherapy, the method comprising:

• (a) obtaining a biological sample from the subject;
• (b) determining a number of expressed genes that are implicated in anti-tumor immune cell signaling/activation in a biological sample;
• (c) determining a number of expressed genes that are implicated in immunosuppression in the biological sample;
• and
• (d) calculating an irScore from the determinations of (b) and (c), wherein the irScore=X(low, medium, or high), wherein X is the number of expressed genes that are implicated in anti-tumor immune cell signaling/activation, and wherein low refers to 1-4 expressed genes that are implicated in immunosuppression, medium refers to 5-9 expressed genes that are implicated in immunosuppression, and high refers to 10 or more expressed genes that are implicated in immunosuppression;
• and
• (e) treating the subject with the immunotherapy where the irScore is at least 5low, 6low, 7low, 8low, 9low, 10low, 11low, 12low, 13low, 14low, 15low, 16low, 17low, 18low, 19low, 20low, 21low, 22low, 23low, 24low, or 25low or greater, 5medium, 6medium, 7medium, 8medium, 9medium, 10medium, 11medium, 12medium, 13medium, 14medium, 15medium, 16medium, 17medium, 18medium, 19medium, 20medium, 21medium, 22medium, 23medium, 24medium, or 25medium or greater, or at least 5high, 6high, 7high, 8high, 9high, 10high, 11high, 12high, 13high, 14high, 15high, 16high, 17high, 18high, 19high, 20high, 21high, 22high, 23high, 24high, or 25high or greater.