Microglia microvesicles contained microRNA-based methods for the diagnosis, prognosis and treatment monitoring of neurological, neurodegenerative and inflammation-based diseases

A method for the in vitro diagnosis of Parkinson's Disease (PD) in an individual, wherein the method comprises the steps:

• a) isolating microglial microvesicles (MVs) from biological fluids obtained from an individual;
• b) collecting the miRNA contained in said MVs;
• c) determining the expression profile of a predetermined set of miRNA, defining a microglial MVs miRNA pattern;
• d) comparing said expression profile to one or several reference expression profiles obtained from at least one healthy individual, or an individual not having Parkinson's Disease (PD);
• and,
• e) treating said individual with a suitable therapy based on the diagnosis of said individual;
• whereby a difference between said determined expression profile and said one or several reference expression profiles is indicative of Parkinson's Disease (PD);
• whereby said microglial MVs miRNA pattern, with respect to said reference pattern, is characterised as follows:
• downregulated miRNA:
• miR-100-5p (SEQ ID 34), miR-152-3p (SEQ ID 35), miR-155-5p (SEQ ID 32), miR-1949 (SEQ ID 27), miR-501-5p (SEQ ID 5), miR-6399 (SEQ ID 21), miR-872-3p (SEQ ID 28);
• upregulated miRNA:
• miR-128-1-5p (SEQ ID 26), miR-16-1-3p (SEQ ID 20), miR-219a-1-3p (SEQ ID 25), miR-222-3p (SEQ ID 36), miR-23a-5p (SEQ ID 23), miR-338-5p (SEQ ID 30), miR-379-5p (SEQ ID 31), miR-450a-5p (SEQ ID 33), miR-582-3p (SEQ ID 29), miR-6240 (SEQ ID 22), miR-92a-1-5p (SEQ ID 24).