Cyclin-dependent kinase substrate
AssigneeSysmex Corporation
InventorsNoriko Yokose, Masatoshi Suganuma, Kenta Noda, Naoki Nishiyama
Life of this patent
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Abstract
Disclosed is a cyclin-dependent kinase substrate including a polypeptide that contains an amino acid sequence represented by formula (1): R1—P (wherein R1 represents a serine residue or a threonine residue, P represents a proline residue, “—” represents a single bond, and the left side represents the N-terminal side), and satisfies the following (a1) and/or (b1): (a1) the second amino acid residue counting from the proline residue toward the N-terminal side in the formula (1) is an aromatic amino acid residue, and/or (b1) at least two amino acid residues from the proline residue toward the C-terminal side in the formula (1) are acidic amino acid residues.
Claims5
A method for measuring the cyclin-dependent kinase activity of a test sample, the method comprising steps (i) and (ii):
- (i) bringing the test sample into contact with a cyclin-dependent kinase substrate;
- and
- (ii) measuring the amount of a substance produced by cyclin-dependent kinase in the test sample, wherein
- the substrate comprises a polypeptide that contains an amino acid sequence represented by formula (1):
- R1—P wherein R1 represents a serine residue or a threonine residue, P represents a proline residue, “—” represents a single bond, and the left side represents the N-terminal side, and satisfies (a1) and/or (b1):
- (a1) the second amino acid residue counting from the proline residue toward the N-terminal side in the formula (1) is an aromatic amino acid residue, and/or
- (b1) at least two amino acid residues from the proline residue toward the C-terminal side in the formula (1) are acidic amino acid residues,
- wherein the substance produced in step (ii) is a modified substrate, and
- wherein the measuring of step (ii) comprises steps (iia)-(iic):
- (iia) mixing the modified substrate with a binding molecule for the modified substrate, to form a complex containing the modified substrate and the binding molecule, wherein the complex is formed within a solution on a solid phase carrier;
- (iib) separating the solid phase carrier on which the complex is formed from the solution;
- and
- (iic) measuring the amount of the modified substrate by measuring the amount of the complex.
A method for measuring the cyclin-dependent kinase activity of a test sample, the method comprising steps (i) and (ii):
- (i) bringing the test sample into contact with a cyclin-dependent kinase substrate;
- and
- (ii) measuring the amount of a substance produced by cyclin-dependent kinase in the test sample, wherein
- the substrate comprises a polypeptide that contains an amino acid sequence represented by formula (1):
- R1—P wherein R1represents a serine residue or a threonine residue, P represents a proline residue, “—” represents a single bond, and the left side represents the N-terminal side, and satisfies (a1) and (b1):
- (a1) the second amino acid residue counting from the proline residue toward the N-terminal side in the formula (1) is an aromatic amino acid residue, and
- (b1) at least two amino acid residues from the proline residue toward the C-terminal side in the formula (1) are acidic amino acid residues,
- wherein the substance produced in step (ii) is a modified substrate, and
- wherein the measuring of step (ii) comprises steps (iia)-(iic):
- (iia) mixing the modified substrate with a binding molecule for the modified substrate, to form a complex containing the modified substrate and the binding molecule, wherein the complex is formed within a solution on a solid phase carrier;
- (iib) separating the solid phase carrier on which the complex is formed from the solution;
- and
- (iic) measuring the amount of the modified substrate by measuring the amount of the complex.
Dependencies are inferred from the claim text.