US 10,851,361 B2Granted

Hemicellulase compositions

AssigneeAjinomoto Co., Inc.

InventorsYusuke Hagiwara, Yasuhiro Mihara, Tomo Takagi, Satoshi Nakamura, Rie Yakushijin

Life of this patent

Estimating the term…

Abstract

Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.

Claims10

Dependent claims

A hemicellulase preparation comprising a first protein and a second protein, wherein the first protein is at least one protein selected from the group consisting of:

(A1) a first protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, wherein said first protein has hemicellulase activity;
(A2) a first protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(A3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, wherein said first protein has hemicellulase activity;
(B1) a first protein comprising the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, wherein said first protein has hemicellulase activity;
(B2) a first protein comprising the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(B3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, wherein said first protein has hemicellulase activity;
(C1) a first protein comprising the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, wherein said first protein has hemicellulase activity;
(C2) a first protein comprising the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(C3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, wherein said first protein has hemicellulase activity;
(D1) a first protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, wherein said first protein has hemicellulase activity;
(D2) a first protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(D3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, wherein said first protein has hemicellulase activity;
(E1) a first protein comprising the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, wherein said first protein has hemicellulase activity;
(E2) a first protein comprising the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
and
(E3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, wherein said first protein has hemicellulase activity;
and
wherein said second protein is at least one protein selected from the group consisting of:
(F1) a second protein comprising the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, wherein said second protein has a property of enhancing hemicellulase activity of said first protein;
(F2) a second protein comprising the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said second protein has a property of enhancing hemicellulase activity of said first protein;
and
(F3) a second protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, wherein said second protein has a property of enhancing hemicellulase activity of said first protein.

52 more elements

An animal feed additive comprising a first protein and a second protein, wherein the first protein is at least one protein selected from the group consisting of:

(A1) a first protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, wherein said first protein has hemicellulase activity;
(A2) a first protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(A3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, wherein said first protein has hemicellulase activity;
(B1) a first protein comprising the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, wherein said first protein has hemicellulase activity;
(B2) a first protein comprising the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(B3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, wherein said first protein has hemicellulase activity;
(C1) a first protein comprising the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, wherein said first protein has hemicellulase activity;
(C2) a first protein comprising the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(C3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, wherein said first protein has hemicellulase activity;
(D1) a first protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, wherein said first protein has hemicellulase activity;
(D2) a first protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(D3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, wherein said first protein has hemicellulase activity;
(E1) a first protein comprising the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, wherein said first protein has hemicellulase activity;
(E2) a first protein comprising the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
and
(E3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, wherein said first protein has hemicellulase activity;
and
wherein said second protein is at least one protein selected from the group consisting of:
(F1) a second protein comprising the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, wherein said second protein has a property of enhancing hemicellulase activity of said first protein;
(F2) a second protein comprising the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said second protein has a property of enhancing hemicellulase activity of said first protein;
and
(F3) a second protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, wherein said second protein has a property of enhancing hemicellulase activity of said first protein.

52 more elements

An animal feed comprising a first protein and a second protein, wherein the first protein is at least one protein selected from the group consisting of:

(A1) a first protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, wherein said first protein has hemicellulase activity;
(A2) a first protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(A3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, wherein said first protein has hemicellulase activity;
(B1) a first protein comprising the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, wherein said first protein has hemicellulase activity;
(B2) a first protein comprising the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(B3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, wherein said first protein has hemicellulase activity;
(C1) a first protein comprising the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, wherein said first protein has hemicellulase activity;
(C2) a first protein comprising the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(C3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, wherein said first protein has hemicellulase activity;
(D1) a first protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, wherein said first protein has hemicellulase activity;
(D2) a first protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(D3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, wherein said first protein has hemicellulase activity;
(E1) a first protein comprising the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, wherein said first protein has hemicellulase activity;
(E2) a first protein comprising the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
and
(E3) a first protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, wherein said first protein has hemicellulase activity;
and
wherein said second protein is at least one protein selected from the group consisting of:
(F1) a second protein comprising the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, wherein said second protein has a property of enhancing hemicellulase activity of said first protein;
(F2) a second protein comprising the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said second protein has a property of enhancing hemicellulase activity of said first protein;
and
(F3) a second protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, wherein said second protein has a property of enhancing hemicellulase activity of said first protein.

52 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/266,542Filed 4 Feb 2019 · continuation
Priority: JP 2016-1545145 Aug 2016
Published: US 2019/0153416 A123 May 2019
Granted: 1 Dec 2020
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 9/2482
Xylanases: endo-1,4-beta-xylanase
A23K 10/14
Obtained by microbiological or biochemical processes: pretreatment of feeding-stuffs with enzymes
A23K 20/189
Organic substances: enzymes
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…

Family

PCT/JP2017/028415Parent of this continuation · filed 4 Aug 2017
US 2019/0153416 A1Pre-grant publication · published 23 May 2019
US 10,851,361 B2This patent

From the US filing history. Foreign family members aren’t listed.

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Abstract

A hemicellulase preparation comprising a first protein and a second protein, wherein the first protein is at least one protein selected from the group consisting of:

(A1) a first protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, wherein said first protein has hemicellulase activity;
(A2) a first protein comprising the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(A3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 535 of SEQ ID NO:
4, positions 1 to 536 of SEQ ID NO:
26, positions 1 to 536 of SEQ ID NO:
28, or positions 1 to 536 of SEQ ID NO:
30, wherein said first protein has hemicellulase activity;
(B1) a first protein comprising the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, wherein said first protein has hemicellulase activity;
(B2) a first protein comprising the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(B3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 448 of SEQ ID NO:
6, wherein said first protein has hemicellulase activity;
(C1) a first protein comprising the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, wherein said first protein has hemicellulase activity;
(C2) a first protein comprising the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(C3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 183 of SEQ ID NO:
9, wherein said first protein has hemicellulase activity;
(D1) a first protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, wherein said first protein has hemicellulase activity;
(D2) a first protein comprising the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
(D3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 527 of SEQ ID NO:
12, positions 1 to 529 of SEQ ID NO:
32, or positions 1 to 390 of SEQ ID NO:
34, wherein said first protein has hemicellulase activity;
(E1) a first protein comprising the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, wherein said first protein has hemicellulase activity;
(E2) a first protein comprising the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said first protein has hemicellulase activity;
and
(E3) a first protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 608 of SEQ ID NO:
14, wherein said first protein has hemicellulase activity;
and
wherein said second protein is at least one protein selected from the group consisting of:
(F1) a second protein comprising the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, wherein said second protein has a property of enhancing hemicellulase activity of said first protein;
(F2) a second protein comprising the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, wherein said second protein has a property of enhancing hemicellulase activity of said first protein;
and
(F3) a second protein comprising an amino acid sequence showing an identity of 97% or higher to the amino acid sequence of positions 1 to 469 of SEQ ID NO:
36, wherein said second protein has a property of enhancing hemicellulase activity of said first protein.

52 more elements

Facts

Application

16/266,542Filed 4 Feb 2019 · continuation

Priority

JP 2016-1545145 Aug 2016

Published

US 2019/0153416 A123 May 2019

Granted

1 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 9/2482
Xylanases: endo-1,4-beta-xylanase
A23K 10/14
Obtained by microbiological or biochemical processes: pretreatment of feeding-stuffs with enzymes
A23K 20/189
Organic substances: enzymes
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…