US 10,851,355 B2 · IsPETase variants

Abstract

Disclosed are a method for preparing crystals of IsPETase protein, a method for screening an IsPETase protein activity regulator and IsPETase variants using a conformation of the protein crystal, a method for screening, IsPETase variants with increased PETase activity, and a method for decomposing PET using the variants. According to exemplary embodiments of the present invention, it is possible to determine a method for effectively preparing a crystal of the IsPETase protein and to obtain the resulting crystal thereof. Further, according to exemplary embodiments of the present invention, it is possible to identify a tertiary structure of the IsPETase from the crystal thereof and to prepare the variant with an increased PETase activity based on this structure. The IsPETase variant may be used effectively in the PET decomposition field.

Claims7

Dependent claims

An IsPETase variant consisting of the amino acid sequence represented by SEQ ID NO:

1, wherein the IsPETase variant includes the amino acid substitution at position 280 of SEQ ID NO:
1 with alanine.

Dependencies are inferred from the claim text.

Facts

Application

16/443,966Filed 18 Jun 2019

Priority

KR 10-2018-00925408 Aug 2018

Published

US 2020/0048621 A113 Feb 2020

Granted

1 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12N 9/18
Acting on ester bonds: carboxylic ester hydrolases
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
C12N
Microorganisms or enzymes

IsPETase variants

Life of this patent

IsPETase variants

Life of this patent

Abstract

Claims7