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US 10,851,351 B2Granted
Biocatalysts for the preparation of hydroxy substituted carbamates
AssigneeCodexis, Inc.
InventorsFabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
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Abstract
The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
Claims8
An engineered ketoreductase polypeptide capable of converting substrate compound (2), tert-butyl(1-oxo-1-phenylhex-5-yn-2-yl)carbamate, to product compound (1), tert-butyl(1-hydroxy-1-phenylhex-5-yn-2-yl)carbamate,
- wherein the ketoreductase polypeptide comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:4 and comprises a substitution X206F/L, further comprising one or more residue differences as compared to SEQ ID NO:4 selected from:
- X7S;
- X17M;
- X17Q;
- X17R;
- X23V;
- X27L;
- X29G;
- X40R;
- X60I;
- X64V;
- X71P;
- X87L;
- X94A;
- X94P;
- X94S;
- X95M;
- X96L;
- X96Y;
- X105G;
- X113I;
- X122A;
- X127R;
- X131S;
- X144V;
- X145L;
- X147I;
- X147L;
- X147Q;
- X150Y;
- X152G;
- X153G;
- X157C;
- X173L;
- X195A;
- X195G;
- X196M;
- X198S;
- X199H;
- X208R;
- X216R;
- X221S;
- X243S;
- X245I;
- X249F;
- X249G;
- and X249Y.
44 more elements
Dependencies are inferred from the claim text.