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US 10,851,350 B1Granted
Bioreactor production of virus from adherent cells
AssigneeGenelux Corporation
InventorsJoseph Cappello, Richard J. Aguilar
Life of this patent
Estimating the term…
Abstract
Methods for producing viruses from adherent cells are provided. The methods include releasing virus from adherent host cells grown in a bioreactor, and purifying released virus by ultrafiltration and/or diafiltration. The methods can be used to manufacture viruses, including for clinical use, at reduced cost relative to conventional virus manufacturing methods.
Claims49
A method for producing a virus, comprising:
- a) culturing host cells, comprising a virus, in a bioreactor, wherein the bioreactor comprises a matrix for growing adherent cells, wherein:
- the matrix is biocompatible and is of a density that entraps cells or is one to which cells adhere, whereby the cells are entrapped in and/or adhere to the matrix;
- and
- the density of the matrix is such that the cells remain entrapped and/or the matrix is one to which cells remain attached under conditions in which cells are lysed and treated to release the virus, and the flow of process culture medium through the matrix is sufficient for cell growth;
- b) treating the cells that are entrapped in and/or adhered to the matrix to lyse them and release the virus into the process medium in the bioreactor, wherein the lysed cells remain adhered to and/or entrapped in the matrix;
- and then
- c) without further treatment of the process medium to remove cells or cell debris, and in only one or two steps, purifying the released virus from the process medium, wherein:
- one step is ultrafiltration or diafiltration;
- and
- two steps are ultrafiltration and diafiltration.
7 more elements
Dependencies are inferred from the claim text.