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US 10,851,348 B2Granted
Proliferative primary human Sertoli cell cultures and their applications
AssigneeMandalMed, Inc.
InventorsConstance M. John, Alpa Mahuvakar
Life of this patent
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Abstract
Technology for the isolation and propagation of primary human Sertoli cells from normal testes tissue, including cultures of proliferative primary human Sertoli cells for research and clinical applications, and a pharmaceutical composition for cell therapy, ex vivo gene therapy, and for the reduction of autoimmune, allograft, and xenograft immune reactions.
Claims4
A culture of cells, comprising:
- A. a culture surface;
- B. a culture medium disposed adjacent to the culture surface;
- and
- C. Sertoli cells isolated from normal testicular tissue of an adult human male plated on the culture surface with a cell density 6.25×103 or fewer cells/cm2;
- whereby at least a portion of the plated Sertoli cells are proliferative.
2 more elements
A method for establishing a culture of cells, comprising the steps of:
- A. providing a culture surface;
- B. providing a culture medium disposed adjacent to the culture surface;
- C. isolating Sertoli cells from normal testicular tissue of an adult human male;
- and
- D. plating the cells on the culture surface at a density of 6.25×103 or fewer cells/cm2,
- whereby at least a portion of the adhered Sertoli cells proliferate,
- thereby establishing a culture-expanded population of proliferative Sertoli cells.
4 more elements
Dependencies are inferred from the claim text.