Altered adherent stromal cells and methods of producing and using same
AssigneePluristem Ltd.
InventorsEytan Abraham, Lior Raviv, Maya Wadamany, Nadav Eshkol
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Abstract
Disclosed herein are altered adherent stromal cells and methods of producing and utilizing same.
Claims21
A method of increasing CCL5 secretion from adherent stromal cells (ASC), said method comprising the step of incubating ASC from placental or adipose tissue in a three-dimensional (3D) culture in a cytokine-containing growth medium, wherein one or more cytokines have been added to said cytokine-containing growth medium, wherein:
- said one or more cytokines comprise Tumor Necrosis Factor alpha (TNF-alpha), wherein the TNF-alpha is present at a concentration between 1-12 nanograms/milliliter,
- said 3D culture is performed in a bioreactor, and
- incubating said ASC in said cytokine-containing growth medium is commenced during exponential phase,
- thereby increasing CCL5 secretion from ASC.
A population of cells, wherein the majority of said population expresses, on a population level, each of CD10, CD29, CD38, and CD40.
A population of cells, wherein the majority of said population expresses, on a population level, each of CD10, CD29, HLA-DR, and CD74.
Dependencies are inferred from the claim text.