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US 10,851,345 B2Granted
Stem cells and decellularized tissue matrix from cord tissue
AssigneeStem Cell Reserve LP
InventorRaymond Mouzannar
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Abstract
Methods and products obtained from the method for isolating and culturing mixed populations of stem cells, making decellularized tissue matrix, making decellularized tissue matrix infused with said mixed populations of stem cells, and methods of stem cell therapy are provided.
Claims4
A biomatrix, said biomatrix obtained by steps consisting essentially of:
- a) obtaining a first intact portion of umbilical cord tissue;
- b) cleaning said first intact portion of umbilical cord tissue with a sterilizing agent;
- c) decellularizing said first intact portion of umbilical cord tissue to produce a decellularized tissue;
- d) cutting said decellularized tissue to a desired shape without removing surface membranes or blood vessels;
- e) incubating said decellularized tissue with serum, plasma or platelet lysate in 0.1% to 5% oxygen environment;
- and
- f) optionally recombining said decellularized tissue from step e with cells.
4 more elements
Dependencies are inferred from the claim text.