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US 10,851,344 B2Granted
Reversible stencils for fabricating micro-tissues
AssigneesThe J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone, The Regents of the University of California
InventorsNathaniel Huebsch, Bruce Conklin, Kevin E. Healy, Peter Loskill
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Abstract
The invention relates to devices, methods, kits, and compositions for in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues.
Claims25
A method of inducing self-assembly of mammalian cells into one or more dog-bone shaped micro-tissues comprising
- seeding the mammalian cells into one or more microwells of a device comprising
- a cell adhesion substrate;
- and
- a removable elastomeric stencil overlay comprising one or more cut-out patterned microwells comprising two or more circular, oval, rectangular, square, V-shaped, or triangular holes, each hole joined to an adjacent hole by a canal;
- and
- culturing the seeded cells within the microwells, to thereby induce self-assembly of the mammalian cells into one or more dog-bone micro-tissues shaped within adjacent holes and along the canal joining the adjacent holes;
- wherein the cell adhesion substrate binds cells within at least the holes of the cut-out pattern.
4 more elements
Dependencies are inferred from the claim text.