Method for producing purified platelets
AssigneeMegakaryon Corporation
InventorsHidenori Hirose, Michiko Ueda
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Abstract
The present invention provides a method for producing purified platelets from a culture of megakaryocytes, comprising a first centrifugal separation step of centrifugally separating the culture at a centrifugal force of 150×g to 550×g, and a second centrifugal separation step of centrifugally separating, at a centrifugal force of 600×g to 4000×g, a liquid component recovered in the first centrifugal separation step.
Claims19
A method for producing purified platelets from a culture of megakaryocytes, comprising:
- removing megakaryocytes from the culture by centrifuging the culture in a first rotatable separation bowl at a centrifugal force of 150×g to 550×g to give a liquid component comprising platelets separated from the megakaryocytes;
- and
- purifying the platelets in the liquid component by centrifuging the liquid component in a second rotatable separation bowl at a centrifugal force of 600×g to 4000×g.
A method for producing a platelet composition having increased positive rates of PAC-1 and CD62p compared to platelet compositions purified by centrifuging in a centrifuge tube, which comprises
- removing megakaryocytes from a culture of megakaryocytes by centrifuging the culture in a first rotatable separation bowl at a centrifugal force of 150×g to 550×g to give a liquid component comprising platelets separated from the megakaryocytes, and
- purifying the platelets in the liquid component by centrifuging the liquid component in a second rotatable separation bowl at a centrifugal force of 600×g to 4000×g.
A method for producing purified platelets from a culture of megakaryocytes, comprising:
- removing megakaryocytes from the culture by centrifuging the culture in a first rotatable separation bowl at a centrifugal force of 150×g to 550×g to give a liquid component comprising platelets separated from the megakaryocytes;
- adding a wash solution to the second rotatable separation bowl and then rotating the second rotatable separation bowl;
- purifying the platelets in the liquid component by centrifuging the liquid component in a second rotatable separation bowl at a centrifugal force of 600×g to 4000×g;
- and
- adding a recovery solution and then rotating the second rotatable separation bowl.
Dependencies are inferred from the claim text.