US 10,851,342 B2 · Method for producing pluripotent stem cells

Abstract

The present invention allows a TET1 protein to be more stably expressed in human pluripotent stem cells than in the past by, inter alia, substituting the second amino acid from the amino terminal of a TET1 protein with a different amino acid. Furthermore, upon differentiation of said pluripotent stem cells, it is possible to quickly eliminate the expression of, inter alia, NANOG, which is an inhibitor of differentiation and promote the expression of factors related to differentiation by introducing a variant TET1 protein to a pluripotent stem cell. The present invention provides a method for manufacturing pluripotent stem cells with increased differentiation potential, and a substance that is useful to said method.

Claims7

Dependent claims

An isolated nucleic acid that encodes a mutant human TET1 protein having the amino acid sequence selected from the group consisting of SEQ ID NO:

2, 4 and 6, and partial sequences thereof having at least amino acids 1 through 613 of SEQ ID NO:
2, 4 and 6, said amino acid sequence incorporating the binding domain at amino acids 585 to 613 and said mutant human TET1 protein having the second amino acid from the N-terminus substituted with another amino acid, wherein said mutant human TETI protein has enhanced stability as compared to the corresponding non-mutant human TETI protein selected from the group consisting of SEQ ID NO:
2, 4 and 6, wherein said enhanced stability is determined by Western blotting.

Dependencies are inferred from the claim text.

Method for producing pluripotent stem cells

Life of this patent

Method for producing pluripotent stem cells

Life of this patent

Abstract

Claims7