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US 10,851,341 B2Granted
Methods and systems for harvesting cells
AssigneePluristem Ltd.
InventorsHarel Kasuto, Nirit Drori-carmi, Barak Zohar
Life of this patent
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Abstract
Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.
Claims20
A method of seeding cells in a 3-dimensional matrix in a chamber of a bioreactor, comprising:
- a) providing a three-dimensional matrix in a fluid within a container, which container is disposed within said chamber;
- b) introducing a composition comprising cells into the container;
- c) vibrating the container in a reciprocating motion relative to said chamber for a period of time at a frequency and amplitude sufficient to mix the cells throughout the matrix;
- and
- d) discontinuing the vibration to permit the cells to attach to the matrix.
2 more elements
Dependencies are inferred from the claim text.