3D polymeric insert to apply uniform electric field in circular cultureware

An insert configured to be inserted into a circular-shaped petri dish for generating a substantially uniform electric field across the petri dish, when the petri dish is filled with a fluid, to establish a salt bridge, the insert comprising:

• a circular-shaped bottom plate configured to be fitted into the circular-shaped petri dish, the bottom plate defining a circular-shaped space of a substantially uniform thickness together with a circular-shaped bottom surface of the petri dish as a channel accepting said fluid when the insert is inserted into the petri dish;
• a side channel vertically erecting from a circular periphery of said bottom plate, the side channel communicating with said circular shaped space defined by the bottom plate so as to accept said fluid;
• and
• a pair of current rectifying chambers each having a generally planar shape connecting to and communicating with the side channel, the current rectifying chambers being diagonally disposed to face each other along a diameter of the circular shaped bottom plate and being line- symmetric with each other with respect to said diameter, each of the current rectifying chambers extending inwardly from a respective portion of a top end of the side channel and having a salt bridge port to accept the fluid and provide the fluid to said circular-shaped space defined by the bottom plate through said side channel so as to establish the salt bridge,
• wherein portions of the side channel other than the portions thereof respectively connecting to the current rectifying chambers each have a generally concave top profile having a lowest point at a center between the pair of current rectifying chambers and are each shaped such that when the salt bridge is established, the circular-shaped space defined by the bottom plate exhibits a substantially uniform electric field in a direction of said diameter in a substantially entire area thereof.