Polymeric BODIPY dyes and methods for using the same
AssigneeBecton, Dickinson and Company
InventorsGlenn P. Bartholomew, Brent S. Gaylord, Lucy Zhao
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Abstract
Polymeric BODIPY dyes including light harvesting BODIPY unit-comprising multichromophores are provided. In some embodiments, the dyes are polymeric tandem dyes that include a light harvesting BODIPY unit-comprising multichromophore and an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. The polymeric tandem dyes may be covalently linked to a specific binding member. Also provided are methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule using compositions including the polymeric tandem dyes. Kits and systems for practicing the subject methods are also provided.
Claims20
A method of evaluating a sample for the presence of a target analyte, the method comprising:
- (a) contacting the sample with a polymeric tandem dye conjugate that specifically binds the target analyte to produce a labelling composition contacted sample, wherein the polymeric tandem dye conjugate comprises a multichromophore described by the formula (II):
- wherein:
- B1 and B2 are each independently a BODIPY unit;
- each M1 and each M2 are independently a π conjugated co-monomer;
- a, b, c, d, e and f are each independently 0, 1 or 2, wherein b+e≥1;
- n and m are independently 0 to 100,000, wherein n+m≥1;
- and
- one L2 group is a terminal group (G1) and the other L2 group is a linked specific binding member for the target analyte;
- and
- (b) assaying the labelling composition contacted sample for the presence of a polymeric tandem dye conjugate-target analyte binding complex to evaluate whether the target analyte is present in the sample.
A method of labelling a target molecule, the method comprising:
- contacting the target molecule with a polymeric tandem dye to produce a labelled target molecule, wherein the polymeric tandem dye comprises:
- a light harvesting BODIPY unit-comprising multichromophore;
- an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith;
- and
- a conjugation tag.
A flow cytometric system, comprising:
- a flow cytometer comprising a flow path;
- a composition in the flow path, wherein the composition comprises:
- a sample;
- and
- a labelled specific binding member comprising:
- a light harvesting BODIPY unit-comprising multichromophore;
- an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith;
- and
- a specific binding member that specifically binds a target analyte and is covalently linked to the multichromophore.
Dependencies are inferred from the claim text.