Carbohydrate content of CTLA4 molecules

A composition comprising CTLA4A29YL104E-Ig molecules comprising:

• (a) an average molar ratio of sialic acid to CTLA4A29YL104E-Ig molecules of at least about 5;
• (b) an average molar ratio of N-acetyl neuraminic acid (NANA) to CTLA4A29YL104E-Ig molecules of from about 5 to about 10;
• (c) an average molar ratio of N-glycolyl neuraminic acid (NGNA) to CTLA4A29YL104E-Ig molecules less than or equal to 1.5;
• (d) an average molar ratio of N-Acetylgalactosamine (GalNAc) to CTLA4A29YL104E-Ig molecules from about 2.7 to about 3.6;
• (e) an average molar ratio of N-Acetylglucosamine (GlcNAc) to CTLA4A29YL104E-Ig molecules from about 24 to about 28;
• (f) an average molar ratio of galactose to CTLA4A29YL104E-Ig molecules from about 9.2 to about 17;
• (g) an average molar ratio of fucose to CTLA4A29YL104E-Ig molecules from about 4.2 to about 7.0;
• (h) an average molar ratio of mannose to CTLA4A29YL104E-Ig molecules from about 10 to about 20;
• and
• (i) less than or equal to 5.0 area percent high molecular weight species as determined by size exclusion chromatography and spectrophotometric detection,
• wherein the CTLA4A29YL104E-Ig molecules comprise one or more CTLA4A29YL104E-Ig polypeptides having the amino acid sequence set forth in SEQ ID NO:
• 4, 11, 12, 13, 14, 15, or 16.