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US 10,850,282 B2Granted
Multiplex PCR chip and multiplex PCR device comprising same
AssigneeNanobiosys Inc.
InventorsSung Woo Kim, Duck Joong Kim, Seung Hyun Jeun
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Abstract
According to one embodiment of the present invention, a multiplex PCR device is disclosed. The multiplex PCR device comprises a multiplex PCR chip simultaneously carrying a plurality of mutually different nucleic acid molecules, and the invention may be characterised in that, attached spaced apart from each other on the multiplex PCR chip, there are a plurality of probes used for hybridization reactions whereby hybridization takes place specifically with mutually different amplified sequences of the nucleic acid molecules.
Claims12
A multiplex polymerase chain reaction (PCR) chip comprising:
- a chamber having a reaction area therein;
- and
- a plurality of probes disposed in the reaction area, each of the plurality of probes being positioned spaced apart from one another,
- wherein the each of the plurality of probes has a configuration to be hybridized with each of different amplified sequences of a plurality of nucleic acid molecules, respectively,
- wherein the probes are marked with the same fluorescent dye having a same color and arranged on a top surface of the reaction area, wherein each of the different amplified sequences of the plurality of nucleic acid molecules is distinguished based on positions of the probes by detecting light passing through the probes,
- wherein the chamber comprises:
- a first plate in a plate shape;
- a second plate arranged on the first plate to include an inflow part, the reaction area, and an outflow part;
- a third plate arranged on the second plate to cover the reaction area, in which the plurality of probes are arranged on an inner surface;
- and
- a plurality of probe fixing parts extending in a direction toward the first plate,
- wherein the multiple PCR chip further comprises a bubble removing part, made of a light transmissive material, and protruded from the inner surface of the third plate in a direction toward the first plate to guide bubbles in the reaction area away from the plurality of probes, which are disposed on the bubble removing part, and
- wherein each of the plurality of probe fixing parts comprises:
- a center part on the bubble removing part accommodating one of the plurality of probes;
- and
- a surrounding part protruded from the center part adjacent to and surrounding the center part.
13 more elements
A multiplex PCR device comprising:
- a multiplex polymerase chain reaction (PCR) chip comprising:
- a chamber having a reaction area therein;
- and
- a plurality of probes disposed in the reaction area, each of the plurality of probes being positioned spaced apart from one another,
- wherein the each of the plurality of probes has a configuration to be hybridized with each of different amplified sequences of a plurality of nucleic acid molecules, respectively,
- wherein the plurality of probes are marked with the same fluorescent dye having a same color and arranged on a top surface of the reaction area, wherein each of the different amplified sequences of the plurality of nucleic acid molecules is distinguished based on positions of the probes by detecting light passing through the probes,
- wherein the chamber comprises:
- a first plate in a plate shape;
- a second plate arranged on the first plate to include an inflow part, the reaction area, and an outflow part;
- a third plate arranged on the second plate to cover the reaction area, in which the plurality of probes are arranged on an inner surface;
- and
- a plurality of probe fixing parts extending in a direction toward the first plate,
- wherein the multiple PCR chip further comprises a bubble removing part, made of a light transmissive material, and protruded from the inner surface of the third plate in a direction toward the first plate to guide bubbles in the reaction area away from the plurality of probes, which are disposed on the bubble removing part, and
- wherein each of the plurality of probe fixing parts comprises:
- a center part on the bubble removing part accommodating one of the plurality of probes;
- and
- a surrounding part protruded from the center part adjacent to and surrounding the center part;
- a light providing part radiating an excitation light toward the plurality of probes in the multiplex PCR chip;
- and
- a light detection part detecting an emission light generated from the plurality of probes by the excitation light,
- wherein the light providing part and the light detection part use a single wavelength light for a detection.
18 more elements
A multiplex PCR device comprising:
- a multiplex polymerase chain reaction (PCR) chip comprising:
- a chamber having a reaction area therein;
- and
- a plurality of probes disposed in the reaction area, each of the plurality of probes being positioned spaced apart from one another,
- wherein the each of the plurality of probes has a configuration to be hybridized with each of different amplified sequences of a plurality of nucleic acid molecules, respectively,
- wherein the plurality of probes are marked with the same fluorescent dye having a same color and arranged on a top surface of the reaction area, wherein each of the different amplified sequences of the plurality of nucleic acid molecules is distinguished based on positions of the probes by detecting light passing through the probes,
- wherein the chamber comprises:
- a first plate in a plate shape;
- a second plate arranged on the first plate to include an inflow part, the reaction area, and an outflow part;
- a third plate arranged on the second plate to cover the reaction area, in which the plurality of probes are arranged on an inner surface;
- and
- a plurality of probe fixing parts extending in a direction toward the first plate,
- wherein the multiple PCR chip further comprises a bubble removing part, made of a light transmissive material, and protruded from the inner surface of the third plate in a direction toward the first plate to guide bubbles in the reaction area away from the plurality of probes, which are disposed on the bubble removing part, and
- wherein each of the plurality of probe fixing parts comprises:
- a center part on the bubble removing part accommodating one of the plurality of probes;
- and
- a surrounding part protruded from the center part adjacent to and surrounding the center part;
- and
- at least one heat block contacting with the multiplex PCR chip and provide heat to the multiplex PCR chip.
16 more elements
Dependencies are inferred from the claim text.