Nucleic acid analysis apparatus

A nucleic acid analysis apparatus with isothermal based amplification, comprising:

• a chamber comprising a cartridge mounted therein;
• a fluid delivery unit connected with the chamber and adapted to transport reagents within the cartridge for sample purification and/or nucleic acid extraction, wherein the fluid delivery unit comprises a plurality of microchannels for connecting with the cartridge and a pump adapted to provide pneumatic force to transport the reagents stored within the cartridge;
• a thermal unit disposed in the chamber and comprising a heater to provide a predefined temperature for nucleic acid amplification;
• a rotational driven unit connected with the chamber and comprising a motor, a driven shaft, and a motion control unit mounted on the driven shaft, wherein the motion control unit comprises a sliding shell and at least one clamping pin protruded out of a bottom surface of the sliding shell, wherein the driven shaft drives the rotation of the clamping pin to clamp and rotate the cartridge, and drives the sliding shell to move downwardly or upwardly to push down or lift up the cartridge, so that the motion control unit is capable of pressing the cartridge during sample purification and/or nucleic acid extraction and rotating the cartridge with a predefined program during nucleic acid amplification and/or detection;
• and
• at least one optical unit disposed on the chamber and comprising at least one light source and at least one optical detector for detection.