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US 10,850,274 B2Granted
Microfluidic assisted perfusion devices
AssigneeThe Regents of the University of California
InventorsShyni Varghese, Aereas Aung, Han Liang Lim
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Abstract
The disclosure provides for microfluidic devices comprising patterned hydrogels with embedded cells or microtissues.
Claims24
A method for in vitro cell culture and screening comprising:
- (a) providing a cellular composition comprising cells in a polymerizable media of gelatin methacrylate, ascorbic acid and a photoinitiator;
- (b) providing a microfluidic device, comprising:
- (i) an inlet channel;
- (ii) an outlet channel;
- (iii) an optically translucent or transparent cell culture chamber fluidly connected between the inlet and outlet, wherein the chamber comprises a biocompatible layer for cell binding having one or more patterned hydrogels of natural polymers which comprise photoreactive side groups;
- (c) flowing the cellular composition from the inlet to the outlet of said microfluidic device such that the cells in the cellular composition flow to, and bind to the one or more patterned hydrogels;
- and
- (d) polymerizing the cellular composition at the one or more patterned hydrogels of natural polymers with UV light to obtain cellular islands.
5 more elements
Dependencies are inferred from the claim text.