Chromatography medium
AssigneePuridify Ltd.
InventorsOliver Hardick, Daniel Gilbert Bracewell, Stewart Dods
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Abstract
The present invention provides a process for preparing a functionalised polymeric chromatography medium, which process comprises (I) providing two or more non-woven sheets stacked one on top of the other, each said sheet comprising one or more polymer nanofibres, (II) simultaneously heating and pressing the stack of sheets to fuse points of contact between the nanofibres of adjacent sheets, and (III) contacting the pressed and heated product with a reagent which functionalises the product of step (II) as a chromatography medium.
Claims14
A process for preparing a functionalised polymeric chromatography medium, which process comprises
- (I) providing a stack of two or more non-woven sheets stacked one on top of the other, each said sheet consisting essentially of one or more polymer nanofibres,
- (II) simultaneously heating and pressing the stack of sheets to fuse points of contact between the nanofibres of adjacent sheets to form a pressed and heated product, and
- (III) contacting the pressed and heated product of step (II) in a batchwise fashion in a plurality of batches with a respective batch reagent associated with each batch of the plurality of batches, said respective batch reagent being same or different in the plurality of batches, to cause the respective batch reagent to be partially retained within the polymer nanofibers and generate the functionalized polymeric chromatography medium,
- wherein a pressure of between 1 kPa to 500 kPa is applied to the stack of sheets in step (II) and wherein the stack of sheets is heated at a temperature between 190° and 220° in step (II), and
- wherein the pressed and heated product is treated to deprotect or activate any functional groups on the one or more polymer nanofibers prior to the step of contacting with the respective batch reagent.
Dependencies are inferred from the claim text.