ELISA detection of urine DEK to predict and diagnose bladder cancer in humans
AssigneeMedical Diagnostic Laboratories, LLC
InventorsAntara Datta, Jason Trama
Life of this patent
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Abstract
The present invention is directed to a method of detecting a DEK protein in a human urine sample using an ELISA assay. Methods and compositions for detection of DEK using mAb 260-6F9F6 (as detection antibody) and mAb 16-2C9C3 (as capture antibody) in human urine are provided herein. Specifically, the ELISA assay utilizes a capture mAb and a detection mAb to yield a high sensitivity of <50 ng/mL. The presence of DEK in urine is useful in predicting or diagnosing the occurrence of bladder cancer in humans.
Claims9
A method of detecting bladder cancer in a human, comprising the steps of:
- (a) obtaining a urine sample from a human suspected of suffering from bladder cancer;
- (b) concentrating said urine sample;
- (c) immobilizing said concentrated urine sample onto a solid surface;
- (d) adding an anti-DEK antibody to said immobilized concentrated urine sample so as to allow a complex formation between DEK isoform 2 protein, and said anti-DEK antibody, said DEK Isoform 2 protein being a protein consisting of SEQ ID NO:2
- wherein said anti-DEK antibody recognizes DEK isoform 2 protein;
- (e) selecting said anti-DEK antibody from the group consisting of a monoclonal antibody 16-2C9C3 or a monoclonal antibody 260-6F9F6;
- and
- (e) detecting said protein-antibody complex, wherein the presence of said protein-antibody complex is indicative of a bladder cancer in said human.
A method of detecting bladder cancer in a human, comprising the steps of:
- (a) obtaining a urine sample from a human suspected of suffering from bladder cancer;
- (b) concentrating said urine sample;
- (c) immobilizing a first anti-DEK antibody onto a solid surface;
- said first anti-DEK antibody is a monoclonal antibody 16-2C9C3;
- (d) adding said concentrated urine sample onto said solid surface having said immobilized first anti-DEK antibody and allowing formation of DEK isoform 2 protein and first anti-DEK antibody complex;
- (e) removing unbound DEK isoform 2 protein;
- said DEK Isoform 2 is a protein consisting of SEQ ID NO:
- 2
- (f) adding a second anti-DEK antibody so as to allow formation of a complex between said bound DEK isoform 2 protein with said second anti-DEK antibody said second anti-DEK antibody is a monoclonal antibody 260-6F9F;
- and
- (g) detecting said bound DEK isoform 2 protein with said second anti-DEK antibody complex, wherein said first anti-DEK antibody and said second anti-DEK antibody recognize a different region of DEK isoform 2 protein, and wherein the presence of said protein-antibody complex is indicative of a bladder cancer in said human.
A method of detecting bladder cancer in a human, comprising the steps of:
- (a) obtaining a urine sample from a human suspected of suffering from bladder cancer;
- (b) concentrating said urine sample;
- (c) immobilizing a first anti-DEK antibody onto a solid surface;
- (d) adding said concentrated urine sample onto said solid surface having said immobilized first anti-DEK antibody and allowing formation of DEK isoform 2 protein and first anti-DEK antibody complex;
- (e) removing unbound DEK isoform 2 protein;
- (f) adding a second anti-DEK antibody so as to allow formation of a complex between said bound DEK isoform 2 protein with said second anti-DEK antibody;
- and
- (g) detecting said bound DEK isoform 2 protein with said second anti-DEK antibody complex, wherein said first anti-DEK antibody and said second anti-DEK antibody recognize a different region of DEK isoform 2 protein, and wherein the presence of said protein-antibody complex is indicative of a bladder cancer in said human;
- wherein the first anti-DEK antibody is a monoclonal antibody 16-2C9C3 and the second anti-DeK antibody is a monoclonal antibody 260-6F9F6.
Dependencies are inferred from the claim text.