Cell extraction using GEITP

A cell extraction system for use with a gradient elution isotachophoresis method, comprising:

• slurrying a mixed-cell sample containing target cells and non-target cells in a trailing electrolyte solution, the trailing electrolyte solution being adapted to migrate slower than the target cells;
• providing a leading electrolyte solution to a separation path in fluid contact with the trailing electrolyte solution, the leading electrolyte solution being adapted to migrate faster than the target cells;
• applying an electric field along the separation path to focus the target cells at the interface between the leading-electrolyte solution and the trailing electrolyte solution;
• extracting the target cells into the separation path;
• and
• expelling the target cells from the separation path into a container.

A cell extraction electrolyte solution for use with a gradient elution isotachophoresis method, comprising:

• a trailing electrolyte solution adapted to migrate slower than target cells while using the gradient elution isotachophoresis method, wherein a mixed-cell sample containing the target cells and non-target cells is slurried in the trailing electrolyte solution prior to cell extraction;
• a leading electrolyte solution adapted to migrate faster than the target cells while using the gradient elution isotachophoresis method, wherein the leading electrolyte solution is provided to a separation path in fluid contact with the trailing electrolyte solution;
• and
• the leading electrolyte solution has a Tris buffer concentration from about 100 mM to about 2000 mM and a tartrate concentration from about 100 mM to about 1000 mM.