Back to results
US 10,843,104 B2Granted
System and process for biopolymer chromatography
AssigneeCytiva Sweden AB
InventorsKarol Lacki, Martin Hall
Life of this patent
Estimating the term…
Abstract
A chromatography system for separation of a biopolymer is described, comprising at least one feed tank, at least one hold tank, at least one elution buffer tank, at least one eluate tank, at least two packed bed chromatography columns and at least one pump and at least one outlet detector both fluidically connected to said each packed bed chromatography column, wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each fluidically connected to the packed bed chromatography columns via a system of valves.
Claims13
A chromatography system for separation of a biopolymer, comprising:
- a group of chromatography columns comprising at least a first column, a second column, and a third column, each respective column of the group of chromatography columns having at least one inlet end and at least one outlet end;
- a system of tanks comprising:
- (i) a feed tank,
- (ii) a group of hold tanks comprising at least three hold tanks,
- (iii) an elution buffer tank, and
- (iv) an eluate tank,
- wherein a first hold tank of the group of hold tanks has a first end connected to a respective inlet end of the first column and a second end connected to a respective outlet end of the third column,
- wherein a second hold tank of the group of hold tanks has one end connected to a respective inlet end of the second column and a second end connected to a respective outlet end of the first column, and
- wherein a third hold tank of the group of hold tanks has a first end connected to a respective inlet end of the third chromatography column and a second end connected to a respective outlet end of the second column,
- a group of pumps, each pump of the group of pump having a first end being directly fluidically connected to a respective inlet end of a respective column of the group of columns, each pump of the group of pumps having a second end directly fluidically connected to a respective hold tank of the group of hold tanks;
- an outlet detector fluidically connected to each column of the group of chromatography columns;
- a system of valves;
- and
- a control unit,
- wherein the control unit is connected to at least one valve of the system of valves, the group of pumps, and the outlet detector,
- convey wherein each hold tank of the group of hold tanks is adapted to receive a fluid from the outlet of a first respective column of the group of columns and convey fluid to an inlet of a second respective column of the group of columns, and
- wherein each hold tank of the group of hold tanks is configured to function as a temporary storage vessel to handle any mismatch flows between the first respective column and the second respective column.
14 more elements
Dependencies are inferred from the claim text.