Method and associated device for rapid detection of target biomolecules with enhanced sensitivity

A rapid detection method of a target biomolecule, comprising:

• contacting a source biological sample comprising the target biomolecule to an ion-exchange medium comprising one or more ligands to capture the target biomolecule and form a captured-target biomolecule, the target biomolecule comprising an antigenic moiety;
• eluting, using an elution buffer having a buffer salt concentration greater than 150 mM, the captured-target biomolecule from the ion-exchange medium as an eluate comprising a concentrated form of the target biomolecule in a solution, wherein a concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the target biomolecule in the source biological sample, and wherein the solution has a salt concentration greater than 150 mM;
• and
• loading the eluate comprising the concentrated form of the target biomolecule in the solution to a rapid diagnostic testing device comprising an antibody, wherein the target biomolecule binds to the antibody under the salt concentration of greater than 150 mM.