Loading patent…
AssigneeJiangnan University
InventorsZhemin Zhou, Long Yu, Li Zhou, Wenjing Cui, Zhongmei Liu, Junling Guo
Estimating the term…
The invention relates to the technical field of bioengineering, and discloses a method for synthesizing L-aspartic acid with maleic acid by whole-cell biocatalysis. In the invention, a recombinant strain co-expressing maleate cis-trans isomerase and L-aspartate lyase is constructed, and engineered and optimized to produce L-aspartic acid from maleic acid with a high conversion rate by whole-cell catalyzing. Relatively inexpensive maleic acid is utilized by the recombinant strain to produce L-aspartic acid, where maleic acid is reacted completely in 40-120 min, there is almost no buildup of the intermediate fumaric acid, and the conversion rate is up to 98% or more.
A recombinant strain co-expressing maleate cis-trans isomerase and L-aspartate lyase, wherein the recombinant strain is produced by expressing, in Escherichia coli as a host, a recombinant expression vector pRSFDuet-1-maiA-aspA constructed with pRSFDuet-1 which is used as an expression vector.
A method for preparing L-aspartic acid, comprising:
Dependencies are inferred from the claim text.